Τίτλος:
Molecular and immunological characterization of Mus a 5 allergen from banana fruit
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Scope Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of β-1,3-glucanase from banana (Musa acuminata), denoted as Mus a 5, were performed Methods and results The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI-TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33451±67Da. Two Mus a 5 isoforms (pI 7.7 and 8.0) were detected by 2D immunoblot with an identical N-terminal sequence. By mass fingerprint, 76 and 83% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74% of patients sensitized to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration-dependent manner Conclusion Mus a 5 is a functional allergen and a candidate for the component-resolved allergy diagnosis of banana allergy. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Συγγραφείς:
Aleksic, I.
Popovic, M.
Dimitrijevic, R.
Andjelkovic, U.
Vassilopoulou, E.
Sinaniotis, A.
Atanaskovic-Markovic, M.
Lindner, B.
Petersen, A.
Papadopoulos, N.G.
Gavrovic-Jankulovic, M.
Περιοδικό:
Molecular Nutrition and Food Research
Λέξεις-κλειδιά:
allergen; CD63 antigen; CD63 protein, human; endo 1,3(4) beta glucanase; ENPP3 protein, human; immunoglobulin E; inorganic pyrophosphatase; isoprotein; phosphodiesterase; plant antigen; vegetable protein, adolescent; adult; amino acid sequence; article; chemistry; female; food allergy; fruit; genetics; human; immunology; isolation and purification; male; metabolism; methodology; molecular cloning; molecular genetics; Musa; skin test; upregulation, Adolescent; Adult; Allergens; Amino Acid Sequence; Antigens, CD63; Antigens, Plant; Cloning, Molecular; Endo-1,3(4)-beta-Glucanase; Female; Food Hypersensitivity; Fruit; Humans; Immunoglobulin E; Male; Molecular Sequence Data; Musa; Phosphoric Diester Hydrolases; Plant Proteins; Protein Isoforms; Pyrophosphatases; Skin Tests; Up-Regulation; Young Adult, Musa acuminata
DOI:
10.1002/mnfr.201100541
