Τίτλος:
The expression of Omental 11β-HSD1 is not increased in severely obese women with metabolic syndrome
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Objective: Plasma cortisol in obese subjects does not differ from that in normoweight subjects. Extra-adrenal cortisol production by 11β- hydroxysteroid dehydrogenase type 1 (11β-HSD1) can result in local hypercortisolemia. The aim of the present study was to examine the role of visceral hypercortisolemia in the development of metabolic syndrome in severe obesity. Methods: Eight lean women during hysterectomy (controls) and 19 severely obese women during bariatric surgery were studied, 8 without metabolic syndrome (OM- group) and 11 with it (OM+ group). Biopsies of omental and subcutaneous fat were performed in the severely obese women during surgery, but only omental biopsies in the controls. Expression of 11β-HSD1, glucocorticoid receptor α (GRα) and glucocorticoid receptor β (GRβ) was evaluated using real-time PCR.Results: Omental 11β-HSD1 expression was different between groups (one-way ANOVA, p < 0.01). Post-hoc analysis revealed that mean omental 11β-HSD1 mRNA levels were higher in the OM- group compared to controls, whereas they were similar when comparing the OM+ group with lean controls. Expression of 11β-HSD1 in subcutaneous fat was not different between OM+ and OM- groups. GRα expression in omental fat did not differ among groups or between omental and subcutaneous fat in severely obese patients. An expression of GRβ was not detected.Conclusion: Contrary to our original hypothesis, omental 11β-HSD1 expression is not increased in the OM+ group. Copyright © 2012 S. Karger GmbH, Freiburg.
Συγγραφείς:
Michalaki, M.
Kyriazopoulou, V.
Antonacopoulou, A.
Koika, V.
Nikolaou, M.
Tsoukas, A.
Kalfarentzos, F.
Vagenakis, A.G.
Voukelatou, G.
Papavassiliou, A.G.
Λέξεις-κλειδιά:
11beta hydroxysteroid dehydrogenase 1; glucocorticoid receptor alpha; glucocorticoid receptor beta; metformin, adult; article; bariatric surgery; clinical article; controlled study; disease severity; female; human; human tissue; hypercortisolism; hysterectomy; metabolic syndrome X; non insulin dependent diabetes mellitus; obesity; peritoneal biopsy; priority journal; protein expression; real time polymerase chain reaction; subcutaneous fat, 11-beta-Hydroxysteroid Dehydrogenase Type 1; Adult; Analysis of Variance; Bariatric Surgery; Biopsy; Female; Humans; Hydrocortisone; Hysterectomy; Intra-Abdominal Fat; Metabolic Syndrome X; Middle Aged; Obesity, Morbid; Real-Time Polymerase Chain Reaction; Receptors, Glucocorticoid; RNA, Messenger; Subcutaneous Fat; Young Adult
