Τίτλος:
Optimization of psoriasis mouse models
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Introduction: Psoriasis, is a common, chronic, autoimmune, inflammatory, relapsing disease, which would benefit from reliable and human-relevant animal models to test drugs pre-clinically and to understand their mechanism of action. Because of its ease of use, convenience and low cost, the imiquimod (IMQ)-induced psoriasis-like model is widely utilized; however, it is not known whether all mouse strains are equivalent and if the hairless mouse is appropriate, so that the imiquimod model can be further optimized. Methods: Under similar experimental conditions, common mouse strains (BALB/c, C57BL/6J, and ApoE) and a new hairless strain (ApoE/SKH-hr2) as well as several inducers (IMQ, IMQ + acetic acid (AcOH) topical and IMQ + AcOH systemic) were compared by clinical, histopathological, biophysical and locomotor activity assessments. Results and discussion: The BALB/c mice yielded an optimal psoriasis-like phenotype with IMQ + AcOH topical treatment, and the corresponding phenotypes for the other mouse strains were C57BL/6J moderate and ApoE mild. In contrast, the ApoE/SKH-hr2 mice, as a result of the absence of a Munro abscess in the histopathology analysis, left doubt about the psoriasis-like acquisition. Locomotor activity of BALB/c mice treated with IMQ, IMQ + AcOH topically and IMQ + AcOH systemically showed decreased distance and rearing coverage and increased immobility with all treatments. Hence, the BALB/c mouse strain appears to be an optimal psoriasis-like model when utilizing IMQ + AcOH topical application. © 2021 Elsevier Inc.
Συγγραφείς:
Karamani, C.
Antoniadou, I.T.
Dimou, A.
Andreou, E.
Kostakis, G.
Sideri, A.
Vitsos, A.
Gkavanozi, A.
Sfiniadakis, I.
Skaltsa, H.
Papaioannou, G.T.
Rallis, M.C.
Maibach, H.
Περιοδικό:
Journal of Pharmacological and Toxicological Methods
Εκδότης:
ELSEVIER SCIENCE INC 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
Λέξεις-κλειδιά:
acetic acid; antidepressant agent; anxiolytic agent; drinking water; imiquimod; imiquimod, animal cell; animal experiment; animal model; animal tissue; apolipoprotein E knockout mouse; Article; Bagg albino mouse; behavior assessment; biophysics; C57BL 6 mouse; cell infiltration; controlled study; cross breeding; disease model; dry skin; epidermal thickness; erythema; histopathology; hyperkeratosis; immobility; inflammatory cell; locomotion; male; mouse; nonhuman; parakeratosis; phenotype; process optimization; psoriasis; Psoriasis Area and Severity Index; rearing; skin abscess; skin water loss; animal; C57BL mouse; disease model; psoriasis; skin, Animals; Disease Models, Animal; Imiquimod; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Psoriasis; Skin
DOI:
10.1016/j.vascn.2021.107054
