Τίτλος:
Isolation of volatile compounds with repellent properties against aedes albopictus (Diptera: Culicidae) using cpc technology
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The present work describes the use of Centrifugal Partition Chromatography (CPC) for the bio-guided isolation of repellent active volatile compounds from essential oils. Five essential oils (EOs) obtained from three Pinus and two Juniperus species were initially analyzed by gas chromatography–mass spectrometry (GC/MS) and evaluated for their repellent properties against Aedes albopictus. The essential oil from needles of P. pinea (PPI) presented the higher activity, showing 82.4% repellency at a dose of 0.2 μL/cm2. The above EO, together with the EO from the fruits of J. oxycedrus subsp. deltoides (JOX), were further analyzed by CPC using the biphasic system n-Heptane/ACN/BuOH in ratio 1.6/1.6/0.2 (v/v/v). The analysis of PPI essential oil resulted in the recovery of (−)-limonene, guaiol and simple mixtures of (−)-limonene/β-pheladrene, while the fractionation of JOX EO led to the recovery of β-myrcene, germacrene-D, and mixtures of α-pinene/β-pinene (ratio 70/30) and α-pinene/germacrene D (ratio 65/45). All isolated compounds and recovered mixtures were tested for their repellent activity. From them, (−)-limonene, guaiol, germacrene-D as well the mixtures of (−)-limonene/β-pheladrene presented significant repellent activity (>97% repellency) against Ae. albopictus. The present methodology could be a valuable tool in the effort to develop potent mosquito repellents which are environmentally friendly. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Συγγραφείς:
Liakakou, A.
Angelis, A.
Papachristos, D.P.
Fokialakis, N.
Michaelakis, A.
Skaltsounis, L.A.
Λέξεις-κλειδιά:
essential oil; insect repellent, Aedes; animal; bioassay; chemistry; chromatography; drug effect; human; isolation and purification; Juniperus; mass fragmentography; pine; procedures; volatilization, Aedes; Animals; Biological Assay; Chromatography; Gas Chromatography-Mass Spectrometry; Humans; Insect Repellents; Juniperus; Oils, Volatile; Pinus; Volatilization
DOI:
10.3390/molecules26113072
