Τίτλος:
Secretory phospholipase a2-iia protein and mrna pools in extracellular vesicles of bronchoalveolar lavage fluid from patients with early acute respiratory distress syndrome: A new perception in the dissemination of inflammation?
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Secretory phospholipase-IIA A2 (sPLA2-IIA) is expressed in a variety of cell types under inflammatory conditions. Its presence in the bronchoalveolar lavage (BAL) fluid of patients with acute respiratory distress syndrome (ARDS) is associated with the severity of the injury. Exosomal type extracellular vesicles, (EVs), are recognized to perform intercellular communication. They may alter the immune status of recipient target cells through cargo shuttling. In this work, we characterized the exosomal type EVs isolated from BAL fluid of patients with early and late ARDS as compared to control/non-ARDS patients, through morphological (confocal and electron microscopy) and biochemical (dynamic light scattering, qRT-PCR, immunoblotting) approaches. We provide evidence for the presence of an sPLA2-IIA-carrying EV pool that coprecipitates with exosomes in the BAL fluid of patients with ARDS. PLA2G2A mRNA was present in all the samples, although more prominently expressed in early ARDS. However, the protein was found only in EVs from early phase ARDS. Under both forms, sPLA2-IIA might be involved in inflammatory responses of recipient lung cells during ARDS. The perception of the association of sPLA2-IIA to the early diagnosis of ARDS or even with a mechanism of development and propagation of lung inflammation can help in the adoption of appropriate and innovative therapeutic strategies. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Συγγραφείς:
Papadopoulos, S.
Kazepidou, E.
Antonelou, M.H.
Leondaritis, G.
Tsapinou, A.
Koulouras, V.P.
Avgeropoulos, A.
Nakos, G.
Lekka, M.E.
Λέξεις-κλειδιά:
messenger RNA; secretory phospholipase A2; secretory phospholipase A2 IIA; unclassified drug, adult respiratory distress syndrome; Article; bronchoalveolar lavage fluid; clinical article; confocal microscopy; controlled study; density gradient centrifugation; electron microscopy; exosome; human; human cell; immunoblotting; pathogenesis; photon correlation spectroscopy; precipitation; protein expression; protein RNA binding; real time polymerase chain reaction; transmission electron microscopy
