Τίτλος:
Preliminary data of a quantitative point of care test for SARS-CoV-2 antibodies from greece
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: Antibody testing is necessary to identify immune individuals in the post-initial wave of the COVID-19 pandemic. Patients and Methods: We prospectively evaluated the performance of a quantitative point-of-care test (POCT) for SARS-CoV-2 antibodies. The patient group (PG) comprised of hospitalized confirmed COVID-19 cases. Asymptomatic healthcare volunteers with negative rRT-PCR were included in the control group (CG). Measurement of IgM and IgG was obtained by dry fluorescence immunoassay. Results: Twenty-six PG (65.9±15.4 years old, male 57.7%) and 18 CG (45.6±10.1 years old, male 33.3%) were included. By manufacturer's cut-off (≥0.04 mIU/ml), sensitivity and specificity were 73.08% and 88.89% for IgM and 88.46% and 33.33% for IgG, respectively. Estimated areas under the ROC curve were 0.907 and 0.848 for IgM and IgG, respectively. Results were improved using a cut-off of IgM ≥0.05 mIU/ml and IgG ≥0.10 mIU/ml. Conclusion: Using stringent cut-off values, SARS-CoV-2 antibody POCT detects immune people and can be used during socioeconomic normalization of communities. © 2020 International Institute of Anticancer Research. All rights reserved.
Συγγραφείς:
FRAGKOU, P.C.
PAPAEVANGELOU, V.
ANTONIADOU, A.
KAVVATHA, D.
PLOUSSI, A.
PANTAZIS, N.
SIRMPILANTZE, T.
PSARRAKIS, C.
POURNARAS, S.A.
TSIODRAS, S.
KELEKIS, A.
Περιοδικό:
In vivo (Athens, Greece)
Εκδότης:
International Institute of Anticancer Research
Λέξεις-κλειδιά:
immunoglobulin G; immunoglobulin M; SARS-CoV-2 antibody; virus RNA; immunoglobulin G; virus antibody, aged; Article; clinical article; controlled study; coronavirus disease 2019; diagnostic test accuracy study; disease severity; female; fluorescence; follow up; Greece; human; immunoassay; male; observational study; point of care testing; preliminary data; prospective study; quantitative diagnosis; real time reverse transcription polymerase chain reaction; RNA extraction; sensitivity and specificity; Severe acute respiratory syndrome coronavirus 2; Betacoronavirus; blood; Coronavirus infection; isolation and purification; middle aged; pandemic; pathogenicity; point of care system; virology; virus pneumonia, Aged; Antibodies, Viral; Betacoronavirus; Coronavirus Infections; Greece; Humans; Immunoglobulin G; Male; Middle Aged; Pandemics; Pneumonia, Viral; Point-of-Care Systems; Sensitivity and Specificity
DOI:
10.21873/invivo.12138
