Τίτλος:
Protective Effects of Pinus halepensis Bark Extract and Nicotine on Cigarette Smoke-induced Oxidative Stress in Keratinocytes
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background/Aim: Cigarette smoke (CS) is a major environmental health threat. The oxidative stress induced by CS on keratinocytes and the possible protective effect of nicotine, its receptor inhibitors, and Pinus halepensis bark extract in relation to known antioxidants were investigated. Materials and Methods: Primary mouse keratinocytes were exposed to cigarette smoke in the presence and absence of Pinus halepensis bark extract (1 μg/ml), rutin (50 μΜ) and ascorbic acid (250 μΜ), nicotine (1 μM) with or without mecamylamine (5 μM) and α-bungarotoxin (0.1 μΜ). Keratinocyte viability and oxidative stress were evaluated by MTT and fluorescence assays. Results: Pinus halepensis bark extract decreased the oxidative stress and increased the viability of keratinocytes, and moreover, these effects were more pronounced compared to the mixture of rutin and Lascorbic acid. Nicotine significantly enhanced the viability potentiation of the beneficial effect induced by Pinus halepensis bark extract. Mecamylamine and α-bungarotoxin showed no specific effect. Conclusion: Pinus halepensis bark extract in combination with nicotine may successfully reverse skin damage induced by cigarette smoke. © 2020 International Institute of Anticancer Research. All rights reserved.
Συγγραφείς:
Pavlou, P.
Antoniadou, I.
Peraki, A.
Vitsos, A.
Dallas, P.
Mostratos, D.
Deliconstantinos, G.
Papaioannou, G.
Grando, S.A.
Rallis, M.
Περιοδικό:
In vivo (Athens, Greece)
Εκδότης:
International Institute of Anticancer Research
Λέξεις-κλειδιά:
alpha bungarotoxin; antioxidant; ascorbic acid; cigarette smoke; mecamylamine; nicotine; Pinus halepensis extract; plant extract; reactive oxygen metabolite; rutoside; unclassified drug; nicotine; plant extract, animal cell; animal tissue; antioxidant activity; Article; cell culture; cell isolation; cell viability; cigarette smoking; comparative study; controlled study; cytokine release; drug potentiation; epidermis cell; fluorescence; fluorescence analysis; keratinocyte; mouse; nonhuman; oxidative stress; pine; Pinus halepensis; animal; bark; keratinocyte; oxidative stress; smoking, Animals; Keratinocytes; Mice; Nicotine; Oxidative Stress; Pinus; Plant Bark; Plant Extracts; Smoking
DOI:
10.21873/invivo.11978
