Τίτλος:
The effect of TISSEEL® on colorectal anastomosis healing process in a diabetic animal experimental model
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background/Aim: Diabetes mellitus is an established risk factor of colorectal anastomosis failure. The purpose of the present study was to evaluate the effect of TISSEEL® in anastomotic healing. Materials and Methods: Forty male, Sprague-Dawley rats were used. Diabetes was induced in half of them by intraperitoneal injection of Streptozotocin, 60 mg/kg. One week after the injection, animals were operated and a 1 cm segment was removed and an end-to-end hand sewn anastomosis was performed. TISSEEL® was applied in each group (diabetic, non-diabetic) following randomization. Results: The pathology analysis revealed improved tissue remodeling in the TISSEEL® group, both for the normoglycemic and the diabetic group. Specifically, the extent of inflammation was decreased (p<0.001), whereas fibroblast and collagen formation were improved (p=0.040 and p=0.008). Neovascularization was also improved (p=0.047). Conclusion: Application of TISSEEL® on colorectal anastomoses improves healing in rats that suffer from severe hyperglycemia. © 2020 International Institute of Anticancer Research. All rights reserved.
Συγγραφείς:
Stergios, K.
Frountzas, M.
Pergialiotis, V.
Korou, L.M.
Kontzoglou, K.
Stefanidis, K.
Nikiteas, N.
Perrea, D.N.
Vaos, G.
Περιοδικό:
In vivo (Athens, Greece)
Εκδότης:
International Institute of Anticancer Research
Λέξεις-κλειδιά:
fibrin glue; fibrin glue; tissue adhesive, animal experiment; animal model; animal tissue; Article; collagen synthesis; colorectal anastomosis; controlled study; diabetes mellitus; end to end anastomosis; experimental model; fibroblast; male; neovascularization (pathology); nonhuman; Sprague Dawley rat; streptozotocin-induced diabetes mellitus; surgical wound; tissue regeneration; wound healing; anastomosis; animal; colon; drug effect; experimental diabetes mellitus; pathophysiology; physiology; procedures; rectum; reproducibility; surgery; wound healing, Anastomosis, Surgical; Animals; Colon; Diabetes Mellitus, Experimental; Fibrin Tissue Adhesive; Male; Models, Animal; Rats, Sprague-Dawley; Rectum; Reproducibility of Results; Tissue Adhesives; Wound Healing
DOI:
10.21873/invivo.11820
