Τίτλος:
DAB2IP expression in abdominal aortic aneurysm: EZH2 and miR-363-3p as potential mediators
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background/Aim: Nine genetic loci have been associated with abdominal aortic aneurysm (AAA) susceptibility, including DAB2IP. This gene is playing a role in apoptosis, cell proliferation and epithelial–to–mesenchymal transition in cancers. This study aimed to elucidate the differential expression levels of DAB2IP in AAA tissues and investigate whether mir-363-3p and EZH2 can be considered as potential mediators of its expression. Materials and Methods: 18 AAA samples and 15 non-aneurysmatic controls were collected. Relative mRNA expression levels of DAB2IP, EZH2 and mir-363-3p were measured using qPCR. Results: DAB2IP was significant up-regulated (~2.29 fold) in AAA tissues, while EZH2 and mir-363-3p were down-regulated (3.28 and 3.62-fold, respectively). A limited negative correlation was found between the DAB2IP and EZH2 expression and between DAB2IP and the mir-363-3p. Conclusion: An increased expression of DAB2IP in AAA tissues was shown. We suggest 2 potential mediators of DAB2IP expression in abdominal aortic aneurysm, EZH2 and mir-363-3p. © 2019 International Institute of Anticancer Research. All rights reserved.
Συγγραφείς:
Legaki, E.
Klonaris, C.
Athanasiadis, D.
Patelis, N.
Sioziou, A.
Liakakos, T.
Gazouli, M.
Περιοδικό:
In vivo (Athens, Greece)
Εκδότης:
International Institute of Anticancer Research
Λέξεις-κλειδιά:
antithrombocytic agent; cholesterol; disabled homolog 2 interacting protein; hydroxymethylglutaryl coenzyme A reductase inhibitor; messenger RNA; microRNA; microRNA 363 3p; protein; transcription factor EZH2; triacylglycerol; unclassified drug; biological marker; DAB2IP protein, human; EZH2 protein, human; guanosine triphosphatase activating protein; microRNA; MIRN363 microRNA, human; transcription factor EZH2, abdominal aortic aneurysm; aged; Article; clinical article; comparative study; controlled study; diabetes mellitus; down regulation; female; human; human tissue; hypertension; male; mRNA expression level; polymerase chain reaction; protein expression; regulator gene; RNA analysis; smoking; upregulation; abdominal aortic aneurysm; comorbidity; gene expression; gene expression regulation; genetics; metabolism; pathology; RNA interference; very elderly, Aged; Aged, 80 and over; Aortic Aneurysm, Abdominal; Biomarkers; Comorbidity; Enhancer of Zeste Homolog 2 Protein; Female; Gene Expression; Gene Expression Regulation; Humans; Male; MicroRNAs; ras GTPase-Activating Proteins; RNA Interference
DOI:
10.21873/invivo.11533
