Τίτλος:
Association between hsa-MIR-30e polymorphisms and sporadic primary hyperparathyroidism risk
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background/Aim: Almost 15% of patients with sporadic primary hyperparathyroidism (sPHPT) present with multiple gland disease (MGD). The aim of this study was to investigate the potential role of two polymorphisms of the hsa-miR-30e, in sPHPT tumorigenesis. Patients and Methods: One-hundred twenty sPHPT patients, 77 presenting a single adenoma and 43 with MGD, and 54 healthy controls were genotyped. The SNPs were identified using the allelespecific PCR methodology, while the hsa-miR-30e expression was analyzed by real-time quantitative reverse transcriptase PCR. Results: Hsa-miR-30e expression was found to be significantly higher in patients with MGD compared to patients with single adenomas (p=0.0019), but no differences were found regarding specific genotype carriers. The genotype frequencies for ss178077483 and rs7556088 were significantly different between patients and healthy controls. Conclusion: Although the polymorphisms cannot be used as biomarkers for the differential diagnosis of MGD, hsa-miR- 30e expression could potentially serve as a biomarker for this purpose. © 2019 International Institute of Anticancer Research. All rights reserved.
Συγγραφείς:
Mizamtsidi, M.
Nastos, K.
Palazzo, F.
Constantinides, V.
Dina, R.
Farenden, M.
Mastorakos, G.
Vassiliou, I.
Gazouli, M.
Περιοδικό:
In vivo (Athens, Greece)
Εκδότης:
International Institute of Anticancer Research
Λέξεις-κλειδιά:
biological marker; microRNA; microRNA 30e; unclassified drug; biological marker; microRNA; MIRN30 microRNA, human, adult; aged; allele specific polymerase chain reaction; Article; controlled study; differential diagnosis; female; gene expression; gene frequency; genetic association; genetic risk; genotype; human; human tissue; major clinical study; male; parathyroid adenoma; primary hyperparathyroidism; real time reverse transcription polymerase chain reaction; retrospective study; risk assessment; single nucleotide polymorphism; adolescent; allele; case control study; gene expression regulation; genetic association study; genetic predisposition; genetics; metabolism; middle aged; odds ratio; primary hyperparathyroidism; young adult, Adolescent; Adult; Aged; Alleles; Biomarkers; Case-Control Studies; Female; Gene Expression Regulation, Neoplastic; Gene Frequency; Genetic Association Studies; Genetic Predisposition to Disease; Genotype; Humans; Hyperparathyroidism, Primary; Male; MicroRNAs; Middle Aged; Odds Ratio; Polymorphism, Single Nucleotide; Retrospective Studies; Young Adult
DOI:
10.21873/invivo.11598
