Τίτλος:
Collagen from the marine sponges Axinella cannabina and Suberites carnosus: Isolation and morphological, biochemical, and biophysical characterization
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
In search of alternative and safer sources of collagen for biomedical applications, the marine demosponges Axinella cannabina and Suberites carnosus, collected from the Aegean and the Ionian Seas, respectively, were comparatively studied for their insoluble collagen, intercellular collagen, and spongin-like collagen content. The isolated collagenous materials were morphologically, physicochemically, and biophysically characterized. Using scanning electron microscopy and transmission electron microscopy the fibrous morphology of the isolated collagens was confirmed, whereas the amino acid analysis, in conjunction with infrared spectroscopy studies, verified the characteristic for the collagen amino acid profile and its secondary structure. Furthermore, the isoelectric point and thermal behavior were determined by titration and differential scanning calorimetry, in combination with circular dichroism spectroscopic studies, respectively. © 2017 by the authors.
Συγγραφείς:
Tziveleka, L.-A.
Ioannou, E.
Tsiourvas, D.
Berillis, P.
Foufa, E.
Roussis, V.
Λέξεις-κλειδιά:
amino acid; collagen; collagen, amino acid analysis; amino acid composition; animal tissue; Article; Axinella cannabina; circular dichroism; comparative study; controlled study; differential scanning calorimetry; infrared spectroscopy; isoelectric point; Middle Pleistocene; nonhuman; physical chemistry; protein isolation; protein secondary structure; protein structure; scanning electron microscopy; sponge (Porifera); structure analysis; Suberites carnosus; thermostability; titrimetry; transmission electron microscopy; animal; aquatic species; Axinella; chemistry; phylogeny; procedures; sponge (Porifera); Suberites, Animals; Aquatic Organisms; Axinella; Collagen; Microscopy, Electron, Scanning; Phylogeny; Porifera; Protein Structure, Secondary; Suberites
