Τίτλος:
Polymorphisms of platelet glycoprotein receptors and cell adhesion molecules in fetuses with fetal growth restriction and their mothers as detected with pyrosequencing
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: Vascular thrombotic tendency may lead to fetal growth restriction (FGR). Altered platelet function and genetic heterogeneity may play a role in this procedure. We investigated whether maternal or fetal genotypic frequencies of genes polymorphisms for certain platelet receptor and cell adhesion molecules are altered in FGR. Materials and Methods: We compared the maternal and fetal genotypic frequencies of single nucleotide polymorphisms (SNPs) in four genes coding for platelet receptors and cell adhesion molecules [integrin alpha subunit 2 (ITGA2 )C807T, integrin subunit beta 3(ITGB3) T1565C, platelet cell adhesion protein 1 (PECAM1) CTG-GTG and selectin P(SELP )A/C]. A total of 32 fetuses with fetal growth restriction and their mothers were matched with 18 normal controls. Using maternal venous blood and umbilical cord blood samples, nucleotide sequences were determined from pyrograms. Genotypic frequencies were calculated and analyzed using appropriate tests and logistic regression. Results: There was no statistical difference in the proportion of heterozygotes or homozygotes for any of the genotypic frequencies between FGR and control groups in mothers or fetuses. Conclusion: Our study demonstrated no association of maternal or fetal ITGA2 C807T SNP, ITGB3 T1565C SNP, PECAM1 CTG-GTG and SELP A/C polymorphisms with FGR.
Συγγραφείς:
Simou, M.
Kouskouni, E.
Vitoratos, N.
Economou, E.
Creatsas, G.
Περιοδικό:
In vivo (Athens, Greece)
Εκδότης:
International Institute of Anticancer Research
Λέξεις-κλειδιά:
alpha integrin; CD31 antigen; cell adhesion molecule; genomic DNA; glycoprotein; integrin alpha subunit 2; integrin subunit beta 3; PADGEM protein; platelet glycoprotein receptor; thrombocyte receptor; unclassified drug; alpha2 integrin; beta3 integrin; CD31 antigen; cell adhesion molecule; fibrinogen receptor; ITGA2B protein, human; ITGB3 protein, human; PADGEM protein; SELP protein, human, adult; Article; comparative study; controlled study; female; fetus; fetus blood sampling; gene frequency; genotype; gestational age; heterozygote; homozygote; human; intrauterine growth retardation; major clinical study; male; maternal blood; molecular pathology; nucleotide sequence; prospective study; pyrosequencing; single nucleotide polymorphism; umbilical cord blood; venous blood; DNA sequence; genetics; intrauterine growth retardation; metabolism; mother; procedures; statistical model, Adult; Antigens, CD31; Cell Adhesion Molecules; Female; Fetal Growth Retardation; Fetus; Gene Frequency; Genotype; Humans; Integrin alpha2; Integrin beta3; Logistic Models; Mothers; P-Selectin; Platelet Membrane Glycoproteins; Polymorphism, Single Nucleotide; Prospective Studies; Sequence Analysis, DNA
DOI:
10.21873/invivo.11052
