Τίτλος:
Microbial biosensors to monitor the encapsulation effectiveness of Doxorubicin in chimeric advanced Drug Delivery Nano Systems: A calorimetric approach
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The release of the anticancer drug doxorubicin (DOX) incorporated in a new drug carrier, namely a chimeric nanosystem formed by liposomes and dendrimers, was studied following the influence of the drug on the growth kinetics of the Lactobacillus helveticus bacterium, that would mimic the intestinal microflora. The bacterial growth was followed at 37 °C by means of Isothermal Titration Calorimetry (ITC) and the method was assessed to monitor the overall effect of the delivered drug obtaining simple objective parameters to define the encapsulation effectiveness of the system, discriminating dose effects even in cases of very low release. Traditional microbiological investigations and in vitro release tests were also performed in parallel for validation. The achieved results suggest that L. helveticus is an excellent candidate as biosensor to assess the sealing effectiveness of these DOX drug carriers through ITC investigations. This approach can be extended for quantitative comparison of drug delivery systems with the same drug inserted in other supramolecular bodies for quantitative comparison. The peculiar results for the DOX drug carrier system investigated, indicate also that, the use of hydrophilic dendrimers in this case, produce a high sealing effect that seems promising in terms of the intestinal flora protection. © 2016 Elsevier B.V.
Συγγραφείς:
Gardikis, K.
Signorelli, M.
Ferrario, C.
Schiraldi, A.
Fortina, M.G.
Hatziantoniou, S.
Demetzos, C.
Fessas, D.
Περιοδικό:
International Journal of Pharmaceutics
Λέξεις-κλειδιά:
dendrimer; doxorubicin; antineoplastic antibiotic; doxorubicin; drug carrier; liposome; nanoparticle; PAMAM G3.5 dendrimer, Article; bacterial growth; biosensor; drug delivery system; drug release; hydrodynamics; in vitro study; isothermal titration calorimetry; Lactobacillus helveticus; nanoencapsulation; nanopharmaceutics; nonhuman; priority journal; calorimetry; chemical phenomena; chemistry; drug delivery system; genetic procedures; Lactobacillus helveticus; metabolism; validation study, Antibiotics, Antineoplastic; Biosensing Techniques; Calorimetry; Dendrimers; Doxorubicin; Drug Carriers; Drug Delivery Systems; Drug Liberation; Hydrophobic and Hydrophilic Interactions; Lactobacillus helveticus; Liposomes; Nanoparticles
DOI:
10.1016/j.ijpharm.2016.11.033
