Τίτλος:
Histamine type 1-receptor activation by low dose of histamine undermines human glomerular slit diaphragm integrity
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Histamine has been reported to decrease the ultrafiltration coefficient, which inversely correlates with glomerular permselectivity, however the mechanism(s) underling this effect have never been investigated. This study aimed to assess whether histamine could exert a direct detrimental effect on podocyte permeability and the possible involvement of two key proteins for the glomerular slit diaphragm (SD) integrity, zonula occludens-1 (ZO-1) and P-cadherin. The effect of histamine (100 pM–1000 nM) on coloured podocytes junctional integrity was evaluated functionally by a transwell assay of monolayer permeability and morphologically by electron microscopy. Histamine receptor (H1-4R) presence was evaluated at both mRNA (RT-PCR) and protein (immunofluorescence) levels. The Kd and Bmax values for [3H]mepyramine were determined by saturation binding analysis; IP1 and cAMP production evoked by histamine were measured by TR-FRET. ZO-1, P-cadherin and vimentin expression was assessed by qRT-PCR and quantitative immunoblotting. Histamine elicited a time- and sigmoidal dose-dependent (maximum effect at 8 h, 10 nM) increase in podocyte paracellular permeability widening the paracellular spaces. Only H1R was predominantly localised to the podocyte membrane. Consistently, histamine elicited a sigmoidal dose-dependent increase in IP1, but not in cAMP. Histamine exposure evoked a concentration-dependent reduction in both ZO-1 and P-cadherin and a parallel induction of vimentin mRNA expression with a maximum effect after 6 h, and protein expression with a maximum effect after 8 h. These effects were prevented by the selective H1R antagonist chlorpheniramine. In conclusion, our data demonstrate that histamine, via the H1R, modifies SD morphological and functional integrity, in part, by decreasing the expression of ZO-1 and P-cadherin. © 2016 Elsevier Ltd
Συγγραφείς:
Veglia, E.
Pini, A.
Moggio, A.
Grange, C.
Premoselli, F.
Miglio, G.
Tiligada, K.
Fantozzi, R.
Chazot, P.L.
Rosa, A.C.
Περιοδικό:
Pharmacology Research and Perspectives
Εκδότης:
INSTAP Academic Press
Λέξεις-κλειδιά:
chlorpheniramine; cyclic AMP; histamine; histamine H1 receptor; histamine H2 receptor; histamine H3 receptor; histamine H4 receptor; inositol phosphate; messenger RNA; P cadherin; protein ZO1; vimentin; cadherin; histamine; histamine agonist; histamine H1 receptor; protein ZO1, Article; controlled study; electron microscopy; gene expression; glomerular slit diaphragm; glomerulus; glomerulus filtration; human; human cell; immunoblotting; immunofluorescence; podocyte; priority journal; protein expression; protein localization; quantitative analysis; reverse transcription polymerase chain reaction; time; cell culture; cell membrane permeability; drug effects; glomerulus; metabolism; pathology; ultrastructure, Cadherins; Cell Membrane Permeability; Cells, Cultured; Histamine; Histamine Agonists; Humans; Kidney Glomerulus; Podocytes; Receptors, Histamine H1; Zonula Occludens-1 Protein
DOI:
10.1016/j.phrs.2016.10.011
