Τίτλος:
Structures, Locations, and Transfer Frequencies of Genetic Elements Conferring High-Level Gentamicin Resistance in Enterococcus faecalis Isolates in Greece
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The elements conferring high-level gentamicin resistance in 64 clinical isolates of Enterococcusfaecalis were characterized by PCR and by restriction enzyme hybridization analysis of genomic and plasmid DNA. There was a strong association between gentamicin resistance and the aac(6′)-aph(2″) gene carried on IS256-based elements with different structures, locations, and transfer characteristics.
Συγγραφείς:
Daikos, G.L.
Bamias, G.
Kattamis, C.
Zervos, M.J.
Chow, J.W.
Christakis, G.
Petrikkos, G.
Triantafyllopoulou, P.
Alexandrou, H.
Syriopoulou, V.
Περιοδικό:
Antimicrobial Agents and Chemotherapy
Λέξεις-κλειδιά:
bacterial DNA; genomic DNA; gentamicin; plasmid DNA; restriction endonuclease, antibiotic resistance; article; bacterial genetics; bacterial strain; bacterium isolate; controlled study; correlation analysis; Enterococcus faecalis; Greece; nonhuman; polymerase chain reaction; priority journal; restriction fragment length polymorphism; transposon, Acetyltransferases; Anti-Bacterial Agents; Chromosomes, Bacterial; Conjugation, Genetic; DNA Transposable Elements; DNA, Bacterial; Drug Resistance, Bacterial; Electrophoresis, Agar Gel; Enterococcus faecalis; Gentamicins; Gram-Positive Bacterial Infections; Greece; Humans; Phosphotransferases (Alcohol Group Acceptor); Plasmids; Reverse Transcriptase Polymerase Chain Reaction
DOI:
10.1128/AAC.47.12.3950-3953.2003
