Τίτλος:
PreDicta chip-based high resolution diagnosis of rhinovirus-induced wheeze
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Rhinovirus (RV) infections are major triggers of acute exacerbations of severe respiratory diseases such as pre-school wheeze, asthma and chronic obstructive pulmonary disease (COPD). The occurrence of numerous RV types is a major challenge for the identification of the culprit virus types and for the improvement of virus type-specific treatment strategies. Here, we develop a chip containing 130 different micro-arrayed RV proteins and peptides and demonstrate in a cohort of 120 pre-school children, most of whom had been hospitalized due to acute wheeze, that it is possible to determine the culprit RV species with a minute blood sample by serology. Importantly, we identify RV-A and RV-C species as giving rise to most severe respiratory symptoms. Thus, we have generated a chip for the serological identification of RV-induced respiratory illness which should be useful for the rational development of preventive and therapeutic strategies targeting the most important RV types. © 2018 The Author(s).
Συγγραφείς:
Niespodziana, K.
Stenberg-Hammar, K.
Megremis, S.
Cabauatan, C.R.
Napora-Wijata, K.
Vacal, P.C.
Gallerano, D.
Lupinek, C.
Ebner, D.
Schlederer, T.
Harwanegg, C.
Söderhäll, C.
Van Hage, M.
Hedlin, G.
Papadopoulos, N.G.
Valenta, R.
Περιοδικό:
Nature Communications
Εκδότης:
Nature Publishing Group
Λέξεις-κλειδιά:
peptide; unclassified drug; viral protein; virus peptide; viral protein 1, rhinovirus, asthma; chronic obstructive pulmonary disease; disease treatment; induced response; peptide; protein; resolution; symptom; viral disease, adolescent; adult; Article; blood sampling; child; female; human; Human rhinovirus A; Human rhinovirus C; major clinical study; male; microarray analysis; nonhuman; Rhinovirus; Rhinovirus infection; virus strain; wheezing; abnormal respiratory sound; asthma; classification; clinical trial; devices; immunology; infant; isolation and purification; picornavirus infection; preschool child; procedures; protein microarray; serology; virology, Rhinovirus, Asthma; Child, Preschool; Female; Humans; Infant; Male; Picornaviridae Infections; Protein Array Analysis; Respiratory Sounds; Rhinovirus; Serologic Tests; Viral Proteins
DOI:
10.1038/s41467-018-04591-0
