Τίτλος:
Direct injection human plasma analysis for the quantification of antihypertensive drugs for therapeutic drug monitoring using hydrophilic interaction liquid chromatography/electrospray ionization mass spectrometry
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The concept of personalized medicine is related to the development of new sensitive, precise and accurate analytical methods for therapeutic drug monitoring. In this article a rapid, sensitive and specific method was developed for the quantification of aliskiren, losartan, valsartan and hydrochlorothiazide in human plasma. Sample preparation was performed by protein precipitation with acetonitrile followed by filtration. All analytes and the internal standard (tiamulin) were separated by hydrophilic interaction liquid chromatography using an X-Bridge-HILIC analytical column (150.0×2.1mm i.d., particle size 3.5μm) under isocratic elution. The mobile phase was composed of a 10% 5mM ammonium formate water solution pH 4.5, adjusted with formic acid, in acetonitrile and pumped at a flow rate of 0.25mLmin-1. The assay was linear over the concentration range of 5-500ngmL-1 for all the analytes. Intermediate precision was less than 5.2% over the tested concentration ranges. The method is the first reported application of HILIC in the analysis antihypertensives in human plasma. With a small sample size (50μL human plasma) and a run time less than 6.0min for each sample the method can be used to support a wide range of clinical studies and therapeutic drug monitoring. © 2015 Elsevier B.V.
Συγγραφείς:
Meimaroglou, S.
Vonaparti, A.
Migias, G.
Gennimata, D.
Poulou, S.
Panderi, I.
Περιοδικό:
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Λέξεις-κλειδιά:
Acetonitrile; Chromatography; Column chromatography; Direct injection; Drug products; Hydrophilicity; Ionization of liquids; Liquid chromatography; Liquids; Mass spectrometry; Particle size; Patient monitoring; Plasma (human); Sampling; Spectrometry, Antihypertensive drugs; Direct injection analysis; Human plasmas; Hydrophilic interaction liquid chromatographies; Therapeutic drug monitoring, Drug interactions, acetonitrile; aliskiren; ammonium formate; formic acid; hydrochlorothiazide; losartan; tiamulin; valsartan; antihypertensive agent, aqueous solution; Article; controlled study; drug blood level; drug monitoring; electrospray mass spectrometry; elution; filtration; flow rate; human; hydrophilic interaction chromatography; limit of quantitation; liquid liquid extraction; particle size; pH; physical chemistry; precipitation; priority journal; quality control; blood; chemical phenomena; drug monitoring; electrospray mass spectrometry; limit of detection; liquid chromatography; procedures; standard; validation study, Antihypertensive Agents; Chromatography, Liquid; Drug Monitoring; Humans; Hydrophobic and Hydrophilic Interactions; Limit of Detection; Reference Standards; Spectrometry, Mass, Electrospray Ionization
DOI:
10.1016/j.jchromb.2015.09.001
