Τίτλος:
Inhibition of Collagenase by Mycosporine-like Amino Acids from Marine Sources
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Matrix metalloproteinases play an important role in extracellular matrix remodeling. Excessive activity of these enzymes can be induced by UV light and leads to skin damage, a process known as photoaging. In this study, we investigated the collagenase inhibition potential of mycosporine-like amino acids, compounds that have been isolated from marine organisms and are known photoprotectants against UV-A and UV-B. For this purpose, the commonly used collagenase assay was optimized and for the first time validated in terms of relationships between enzyme-substrate concentrations, temperature, incubation time, and enzyme stability. Three compounds were isolated from the marine red algae Porphyra sp. and Palmaria palmata, and evaluated for their inhibitory properties against Chlostridium histolyticum collagenase. A dose-dependent, but very moderate, inhibition was observed for all substances and IC50 values of 104.0μM for shinorine, 105.9μM for porphyra, and 158.9μM for palythine were determined. Additionally, computer-aided docking models suggested that the mycosporine-like amino acids binding to the active site of the enzyme is a competitive inhibition. © Georg Thieme Verlag KG Stuttgart, New York .
Συγγραφείς:
Hartmann, A.
Gostner, J.
Fuchs, J.E.
Chaita, E.
Aligiannis, N.
Skaltsounis, L.
Ganzera, M.
Εκδότης:
Georg Thieme Verlag
Λέξεις-κλειδιά:
amino acid derivative; amino acid porphyra; clostridiopeptidase A; mycosporine like amino acid; palythine; shinorine; unclassified drug; amino acid; clostridiopeptidase A; cyclohexanol derivative; cyclohexanone derivative; cyclohexylamine derivative; glycine; matrix metalloproteinase inhibitor; palythine; shinorine, Article; Clostridium histolyticum; drug structure; enzyme inhibition; enzyme stability; enzyme substrate; high throughput screening; IC50; incubation time; marine species; molecular model; Palmaria palmata; Porphyra; analogs and derivatives; antagonists and inhibitors; aquatic species; chemistry; dose response; metabolism; Porphyra; preclinical study; procedures; red alga; reproducibility; temperature, algae; Palmaria palmata; Porphyra; Porphyra sp.; Rhodophyta, Amino Acids; Aquatic Organisms; Cyclohexanols; Cyclohexanones; Cyclohexylamines; Dose-Response Relationship, Drug; Drug Evaluation, Preclinical; Enzyme Stability; Glycine; Inhibitory Concentration 50; Matrix Metalloproteinase Inhibitors; Microbial Collagenase; Porphyra; Reproducibility of Results; Rhodophyta; Temperature
DOI:
10.1055/s-0035-1546105
