Τίτλος:
Pre-Column Derivatization HPLC Procedure for the Quantitation of Aluminium Chlorohydrate in Antiperspirant Creams Using Quercetin as Chromogenic Reagent
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
This article describes the development and validation of a selective high-performance liquid chromatography method that allows, after liquid–liquid extraction and pre-column derivatization reaction with quercetin, the quantification of aluminium chlorohydrate in antiperspirant creams. Chromatographic separation was achieved on an XTerra MS C18 analytical column (150 × 3.0 mm i.d., particle size 5 μm) using a mobile phase of acetonitrile:water (15:85, v/v) containing 0.08 % trifluoroacetic acid at a flow rate of 0.30 mL min−1. Ultraviolet spectrophotometric detection at 415 nm was used. The assay was linear over a concentration range of 3.7–30.6 μg mL−1 for aluminium with a limit of quantitation of 3.74 μg mL−1. Quality control samples (4.4, 17.1 and 30.6 μg mL−1) in five replicates from five different runs of analysis demonstrated intra-assay precision (% coefficient of variation <3.8 %), inter-assay precision (% coefficient of variation <5.4 %) and an overall accuracy (% recovery) between 96 and 101 %. The method was used to quantify aluminium in antiperspirant creams containing 11.0, 13.0 and 16.0 % (w/w) aluminium chlorohydrate, respectively. © 2014, The Author(s).
Συγγραφείς:
Kalogria, E.
Varvaresou, A.
Papageorgiou, S.
Protopapa, E.
Tsaknis, I.
Matikas, A.
Panderi, I.
Περιοδικό:
Chromatographia
Εκδότης:
Friedr. Vieweg und Sohn Verlags GmbH
Λέξεις-κλειδιά:
acetonitrile; aluminum hydroxide; aluminum hydroxychloride; ammonium acetate; antiperspirant agent; buffer; methanol; quercetin; trifluoroacetic acid, accuracy; Article; complex formation; cosmetic industry; cream; derivatization; high performance liquid chromatography; limit of detection; limit of quantitation; particle size; pH; pre column derivatization high performance liquid chromatography; water content
DOI:
10.1007/s10337-014-2722-9
