Τίτλος:
Novel inverse binding mode of indirubin derivatives yields improved selectivity for DYRK kinases
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
DYRK kinases are involved in alternative pre-mRNA splicing as well as in neuropathological states such as Alzheimer's disease and Down syndrome. In this study, we present the design, synthesis, and biological evaluation of indirubins as DYRK inhibitors with enhanced selectivity. Modifications of the bis-indole included polar or acidic functionalities at positions 5′ and 6′ and a bromine or a trifluoromethyl group at position 7, affording analogues that possess high activity and pronounced specificity. Compound 6i carrying a 5′-carboxylate moiety demonstrated the best inhibitory profile. A novel inverse binding mode, which forms the basis for the improved selectivity, was suggested by molecular modeling and confirmed by determining the crystal structure of DYRK2 in complex with 6i. Structure-activity relationships were further established, including a thermodynamic analysis of binding site water molecules, offering a structural explanation for the selective DYRK inhibition. © 2012 American Chemical Society.
Συγγραφείς:
Myrianthopoulos, V.
Kritsanida, M.
Gaboriaud-Kolar, N.
Magiatis, P.
Ferandin, Y.
Durieu, E.
Lozach, O.
Cappel, D.
Soundararajan, M.
Filippakopoulos, P.
Sherman, W.
Knapp, S.
Meijer, L.
Mikros, E.
Skaltsounis, A.-L.
Περιοδικό:
ACS Medicinal Chemistry Letters
Λέξεις-κλειδιά:
bromine; carboxylic acid derivative; dual specificity tyrosine phosphorylation regulated kinase; indirubin; indole derivative; protein kinase; unclassified drug, article; binding affinity; binding site; crystal structure; drug binding; drug design; drug screening; drug synthesis; molecular model; priority journal; structure activity relation; thermodynamics
