Περίληψη:
Argonaute 2 (AGO2) is an indispensable component of the RNA-induced
silencing complex, operating at the translational or posttranscriptional
level. It is compartmentalized into structures such as GW- and P-bodies,
stress granules and adherens junctions as well as the midbody. Here we
show using immunofluorescence, image and bioinformatic analysis and
cytogenetics that AGO2 also resides in membrane protrusions such as
open- and close-ended tubes. The latter are cytokinetic bridges where
AGO2 colocalizes at the midbody arms with cytoskeletal components such
as alpha-Tau ubulin and Aurora B, and various kinases. AGO2,
phosphorylated on serine 387, is located together with Dicer at the
midbody ring in a manner dependent on p38 MAPK activity. We further show
that AGO2 is stress sensitive and important to ensure the proper
chromosome segregation and cytokinetic fidelity. We suggest that AGO2 is
part of a regulatory mechanism triggered by cytokinetic stress to
generate the appropriate micro-environment for local transcript
homeostasis. Pantazopoulou et al. find that AGO2 resides in open-ended
tunneling nanotubes and close-ended cytokinetic bridges. At the latter
location, AGO2 colocalizes with cell division components and the authors
show that AGO2 depletion impairs cell division fidelity.
Συγγραφείς:
Pantazopoulou, Vasiliki I.
Delis, Anastasios D.
Georgiou, Stella
and Pagakis, Stamatis N.
Filippa, Vicky
Dragona, Eleni and
Kloukina, Ismini
Chatzitheodoridis, Elias
Trebicka, Jonel and
Velentzas, Athanassios D.
Thiele, Maja
Gagos, Sarantis and
Thanos, Dimitris
Tseleni-Balafouta, Sofia
Stravopodis, Dimitrios
J.
Anastasiadou, Ema
