Τίτλος:
Adiponectin, Leptin, and IGF-1 Are Useful Diagnostic and Stratification
Biomarkers of NAFLD
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: Nonalcoholic fatty liver disease (NAFLD) is the most common
chronic liver disease where liver biopsy remains the gold standard for
diagnosis. Here we aimed to evaluate the role of circulating
adiponectin, leptin, and insulin-like growth factor 1 (IGF-1) levels as
non-invasive NAFLD biomarkers and assess their correlation with the
metabolome. Materials and Methods: Leptin, adiponectin, and IGF-1 serum
levels were measured by ELISA in two independent cohorts of
biopsy-proven obese NAFLD patients and healthy-liver controls
(discovery: 38 NAFLD, 13 controls; validation: 194 NAFLD, 31 controls)
and correlated with clinical data, histology, genetic parameters, and
serum metabolomics. Results: In both cohorts, leptin increased in NAFLD
vs. controls (discovery: AUROC 0.88; validation: AUROC 0.83; p <
0.0001). The leptin levels were similar between obese and non-obese
healthy controls, suggesting that obesity is not a confounding factor.
In the discovery cohort, adiponectin was lower in non-alcoholic
steatohepatitis (NASH) vs. non-alcoholic fatty liver (AUROC 0.87; p <
0.0001). For the validation cohort, significance was attained for
homozygous for PNPLA3 allele c.444C (AUROC 0.63; p < 0.05). Combining
adiponectin with specific serum lipids improved the assay performance
(AUROC 0.80; p < 0.0001). For the validation cohort, IGF-1 was lower
with advanced fibrosis (AUROC 0.67, p < 0.05), but combination with
international normalized ratio (INR) and ferritin increased the assay
performance (AUROC 0.81; p < 0.01). Conclusion: Serum leptin
discriminates NAFLD, and adiponectin combined with specific lipids
stratifies NASH. IGF-1, INR, and ferritin distinguish advanced fibrosis.
Συγγραφείς:
Marques, Vanda
Afonso, Marta B.
Bierig, Nina
Duarte-Ramos,
Filipa
Santos-Laso, Alvaro
Jimenez-Aguero, Raul
Eizaguirre,
Emma
Bujanda, Luis
Pareja, Maria J.
Luis, Rita
Costa,
Adilia
Machado, V, Mariana
Alonso, Cristina
Arretxe, Enara
and Alustiza, Jose M.
Krawczyk, Marcin
Lammert, Frank and
Tiniakos, Dina G.
Flehmig, Bertram
Cortez-Pinto, Helena and
Banales, Jesus M.
Castro, Rui E.
Normann, Andrea
Rodrigues,
Cecilia M. P.
Περιοδικό:
Frontiers in Cardiovascular Medicine
Εκδότης:
Frontiers Media SA
Λέξεις-κλειδιά:
adiponectin; circulating biomarkers; fibrosis; IGF-1; leptin; lipid
metabolism; NAFLD
DOI:
10.3389/fmed.2021.683250
