Τίτλος:
The O-Linked N-Acetylglucosamine Containing Epitope H (O-GlcNAcH) is
Upregulated in the Trophoblastic and Downregulated in the Fibroblastic
Cells in Missed Miscarriage Human Chorionic Villi With Simple Hydropic
Degeneration
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Epitope H contains an O-linked N-acetylglucosamine (O-GlcNAcH) residue
in a specific conformation and/or environment recognized by the mouse
monoclonal antibody H. O-GlcNAcH is present in several types of cells
and in several polypeptides, including cytokeratin 8 and vimentin, on
the latter in cells under stress. In the present work, we examined the
expression of the O-GlcNAcH in 60 cases of endometrial curettings from
missed miscarriage cases containing normal and simple hydropic
degenerated chorionic villi in each case, using monoclonal antibody H
and indirect immunoperoxidase and Western blot immunoblot. In all cases
examined the expression of the O-GlcNAcH was cytoplasmic as follows: (1)
syncytiotrophoblastic cells showed very low expression in chorionic
villi (CV) with nonhydropic degeneration (NHD) and high expression in
hydropic degenerated (HD) CV; (2) cytotrophoblastic cells showed low
expression in CV with NHD and high expression in HD CV; (3) fibroblastic
cells showed high expression in CV with NHD and very low expression in
HD CV; (4) histiocytes showed very low expression in both types of CV;
(5) endothelial cells showed high expression in both types of CV. An
immunoblot of CV from one case of a legal abortion from a normal
first-trimester pregnancy showed 5 polypeptides with 118.5, 106.3, 85,
53, and 36.7 kD bearing the epitope H and the 53 kD corresponded to
cytokeratin 8. The expression of the O-GlcNAcH is upregulated in the
trophoblastic cells and downregulated in the fibroblastic cells in the
HD CV in comparison to the NHD CV.
Συγγραφείς:
Nikolaou, Michail A.
Drosos, Yiannis
Havaki, Sophia and
Arvanitis, Dimitrios
Sotiriou, Sotirios
Vassiou, Katerina and
Zibis, Aristeidis
Arvanitis, Leonidas D.
Περιοδικό:
International Journal of Gynecological Pathology
Εκδότης:
Lippincott, Williams & Wilkins
Λέξεις-κλειδιά:
Epitope H; O-GlcNAcH; O-GlcNAc; Chorionic villi; Hydropic degeneration
DOI:
10.1097/PGP.0000000000000693
