Περίληψη:
Advancing age has a negative impact on female fertility. As implantation
rates decline during the normal maternal life course, age-related,
embryonic factors are altered and our inability to monitor these factors
in an unbiased genome-wide manner in vivo has severely limited our
understanding of early human embryo development and implantation. Our
high-throughput methodology uses trophectoderm samples representing the
full spectrum of maternal reproductive ages with embryo implantation
potential examined in relation to trophectoderm transcriptome dynamics
and reproductive maternal age. Potential embryo-endometrial interactions
were tested using trophectoderm sampled from young women, with the
receptive uterine environment representing the most `fertile'
environment for successful embryo implantation. Potential roles for
extracellular exosomes, embryonic metabolism and regulation of apoptosis
were revealed. These biomarkers are consistent with embryo-endometrial
crosstalk/developmental competency, serving as a mediator for successful
implantation. Our data opens the door to developing a diagnostic test
for predicting implantation success in women undergoing fertility
treatment.
Συγγραφείς:
Ntostis, Panagiotis
Swanson, Grace
Kokkali, Georgia
Iles,
David
Huntriss, John
Pantou, Agni
Tzetis, Maria
Pantos,
Konstantinos
Picton, Helen M.
Krawetz, Stephen A.
Miller,
David
