Τίτλος:
The NADPH Oxidase A of Verticillium dahliae Is Essential for
Pathogenicity, Normal Development, and Stress Tolerance, and It
Interacts with Yap1 to Regulate Redox Homeostasis
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Maintenance of redox homeostasis is vital for aerobic organisms and
particularly relevant to plant pathogens. A balance is required between
their endogenous ROS production, which is important for their
development and pathogenicity, and host-derived oxidative stress.
Endogenous ROS in fungi are generated by membrane-bound NADPH oxidase
(NOX) complexes and the mitochondrial respiratory chain, while
transcription factor Yap1 is a major regulator of the antioxidant
response. Here, we investigated the roles of NoxA and Yap1 in
fundamental biological processes of the important plant pathogen
Verticillium dahliae. Deletion of noxA impaired growth and
morphogenesis, compromised formation of hyphopodia, diminished
penetration ability and pathogenicity, increased sensitivity against
antifungal agents, and dysregulated expression of antioxidant genes. On
the other hand, deletion of yap1 resulted in defects in conidial and
microsclerotia formation, increased sensitivity against oxidative
stress, and down-regulated antioxidant genes. Localized accumulation of
ROS was observed before conidial fusion and during the heterokaryon
incompatibility reaction upon nonself fusion. The frequency of inviable
fusions was not affected by the deletion of Yap1. Analysis of a double
knockout mutant revealed an epistatic relationship between noxA and
yap1. Our results collectively reveal instrumental roles of NoxA and ROS
homeostasis in the biology of V. dahliae.
Συγγραφείς:
Vangalis, Vasileios
Papaioannou, Ioannis A.
Markakis, Emmanouil
A.
Knop, Michael
Typas, Milton A.
Περιοδικό:
JOURNAL OF FUNGI
Λέξεις-κλειδιά:
antioxidant response; conidial anastomosis tubes (CATs); heterokaryon
incompatibility; oxidative stress; pathogenicity; reactive oxygen
species (ROS); redox homeostasis
