Τίτλος:
Chemical and Biological Characterization of the Anticancer Potency of
Salvia fruticosa in a Model of Human Malignant Melanoma
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Malignant melanoma is one of the most aggressive types of skin cancer
with an increasing incidence worldwide. Thus, the development of
innovative therapeutic approaches is of great importance. Salvia
fruticosa (SF) is known for its anticancer properties and in this
context, we aimed to investigate its potential anti-melanoma activity in
an in vitro model of human malignant melanoma. Cytotoxicity was assessed
through a colorimetric-based sulforhodamine-B (SRB) assay in primary
malignant melanoma (A375), non-malignant melanoma epidermoid carcinoma
(A431) and non-tumorigenic melanocyte neighbouring keratinocyte (HaCaT)
cells. Among eight (8) different fractions of S. fruticosa extracts
(SF1-SF8) tested, SF3 was found to possess significant cytotoxic
activity against A375 cells, while A431 and HaCaT cells remained
relatively resistant or exerted no cytotoxicity, respectively. In
addition, the total phenolic (Folin-Ciocalteu assay) and total flavonoid
content of SF extracts was estimated, whereas the antioxidant capacity
was measured via the inhibition of tert-butyl hydroperoxide-induced
lipid peroxidation and protein oxidation levels. Finally, apoptotic cell
death was assessed by utilizing a commercially available kit for the
activation of caspases - 3, - 8 and - 9. In conclusion, the
anti-melanoma properties of SF3 involve the induction of both extrinsic
and intrinsic apoptotic pathway(s), as evidenced by the increased
activity levels of caspases - 8, and - 9, respectively.
Συγγραφείς:
Kyriakou, Sotiris
Tragkola, Venetia
Plioukas, Michael and
Anestopoulos, Ioannis
Chatzopoulou, Paschalina S.
Sarrou, Eirini
and Trafalis, Dimitrios T.
Deligiorgi, Maria V.
Franco, Rodrigo
and Pappa, Aglaia
Panayiotidis, Mihalis I.
Λέξεις-κλειδιά:
Salvia fruticosa; melanoma; oxidative stress; flavonoids; phenolics;
apoptosis
DOI:
10.3390/plants10112472
