Τίτλος:
Deciphering the Relationship Between Free and Vesicular Hemoglobin in
Stored Red Blood Cell Units
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Red blood cells (RBCs) release hemoglobin (Hb)-containing extracellular
vesicles (EVs) throughout their lifespan in the circulation, and
especially during senescence, by spleen-facilitated vesiculation of
their membrane. During ex vivo aging under blood bank conditions, the
RBCs lose Hb, both in soluble form and inside EVs that accumulate as a
part of storage lesion in the supernatant of the unit. Spontaneous
hemolysis and vesiculation are increasingly promoted by the storage
duration, but little is known about any physiological linkage between
them. In the present study, we measured the levels of total
extracellular and EV-enclosed Hb (EV-Hb) in units of whole blood (n =
36) or packed RBCs stored in either CPDA-1 (n = 99) or in CPD-SAGM
additive solution (n = 46), in early, middle, and late storage. The
spectrophotometry data were subjected to statistical analysis to detect
possible correlation(s) between storage hemolysis and EV-Hb, as well as
the threshold (if any) that determines the area of this dynamic
association. It seems that the percentage of EV-Hb is negatively
associated with hemolysis levels from middle storage onward by showing
low to moderate correlation profiles in all strategies under
investigation. Moreover, 0.17% storage hemolysis was determined as the
potential cut-off, above which this inverse correlation is evident in
non-leukoreduced CPDA units. Notably, RBC units with hemolysis levels >
0.17% are characterized by higher percentage of nanovesicles (<100 nm)
over typical microvesicles (100-400 nm) compared with the lower
hemolysis counterparts. Our results suggest an ordered loss of Hb during
RBC accelerated aging that might fuel targeted research to elucidate its
mechanistic basis.
Συγγραφείς:
Tzounakas, Vassilis L.
Anastasiadi, Alkmini T.
Lekka, Marilena
E.
Papageorgiou, Effie G.
Stamoulis, Konstantinos and
Papassideri, Issidora S.
Kriebardis, Anastasios G.
Antonelou,
Marianna H.
Περιοδικό:
FRONTIERS IN PHYSIOLOGY
Εκδότης:
Frontiers Media SA
Λέξεις-κλειδιά:
hemolysis; red blood cells; storage lesion; extracellular vesicles; size
distribution
DOI:
10.3389/fphys.2022.840995
