Τίτλος:
Comparison between TEWL and laser scanning microscopy measurements for the in vivo characterization of the human epidermal barrier
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The analysis of the skin barrier properties is important in various fields of medical treatment and cosmetology. The development and improvement of topically applied substances require an objective analysis of the skin barrier characteristics. Transepidermal water loss (TEWL) measurement is the standard method to characterize epidermal barrier function. The most important disadvantage of this method though, is that it can be affected by different exogenous and endogenous factors, e.g. water content of the applied formulation and room temperature. In the present study, TEWL measurements are compared to laser scanning microscopic (LSM) measurements, concerning the use of these two methods for the non-invasive in vivo characterization of the epidermal barrier function. The investigations were performed prior and subsequent to treatment of dry skin with a gel mixture, developed for skin treatment after radiotherapy for cancer. The present results indicate that in vivo laser scanning microscopy is an appropriate method for the characterization of the skin barrier structure without interference by external factors. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Συγγραφείς:
Vergou, T.
Schanzer, S.
Richter, H.
Pels, R.
Thiede, G.
Patzelt, A.
Meinke, M.C.
Sterry, W.
Fluhr, J.W.
Lademann, J.
Περιοδικό:
Journal of Biophotonics
Λέξεις-κλειδιά:
Barrier properties; Barrier structures; Dry skin; Endogenous factors; Epidermal barrier; External factors; Gel mixture; In-vivo; Laser scanning; Laser scanning microscopy; Medical treatment; Non-invasive; Objective analysis; Room temperature; Standard method; Transepidermal water loss, Characterization; Dermatology; Scanning, Laser applications, oil; water, adult; article; chemistry; comparative study; confocal microscopy; epidermis; female; functions of the skin and its appendages; gel; human; laser; methodology; middle aged; pathology; skin; thermoregulation, Adult; Epidermis; Female; Gels; Humans; Lasers; Microscopy, Confocal; Middle Aged; Oils; Research Design; Skin; Skin Physiological Phenomena; Water; Water Loss, Insensible
DOI:
10.1002/jbio.201100067
