Τίτλος:
Effects of bioactive glass and β-TCP containing three-dimensional laser sintered polyetheretherketone composites on osteoblasts in vitro
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Because of their excellent physical properties nonresorbable thermoplastic polymers have become more important for the field of reconstructive surgery. Aim of the present study was to investigate the effects of laser sintered polyetheretherketone (PEEK) with incorporated osteoconductive and bioactive bone substitution materials on osteoblasts in vitro. Human osteoblasts (hFOB 1.19) were seeded onto laser sintered PEEK samples containing nano-sized carbon black, β-tricalciumphosphate (β-TCP), and bioactive glass 45S5. Osteoblasts were investigated for cell viability, cell proliferation and cell morphology. A constant proliferation of osteoblasts could be observed on all samples with the highest values for bioactive glass containing samples at day 7 (OD 1.76 ± 0.22) and day 14 (QD 3.75 ± 0.31) and lowest values for β-TCP containing probes throughout the study compared with the PEEK pure control group. Highest cell viability was observed for Bioglass containing probes (95.5 ± 3.32)% whereas osteoblasts seeded on β-TCP containing probes showed reduced viability (84.4 ± 4.32)%. Laser sintered PEEK implants seem to be attractive candidates for use as bone substitutes for reconstructive surgery because of their biocompatibility, individual shape, and the possibility of compounding bioinert polymer powder with osteoconductive and bioactive materials which might benefit bone formation in vivo. © 2008 Wiley Periodicals, Inc.
Συγγραφείς:
Von Wilmowsky, C.
Vairaktaris, E.
Pohle, D.
Rechtenwald, T.
Lutz, R.
Münstedt, H.
Koller, G.
Schmidt, M.
Neukam, F.W.
Schlegel, K.A.
Nkenke, E.
Περιοδικό:
Journal of Biomedical Materials Research Part A
Λέξεις-κλειδιά:
ABS resins; Biocompatibility; Bone; Carbon black; Cell proliferation; Cells; Compounding (chemical); Cytology; Glass; Laser heating; Laser surgery; Lasers; Polymeric glass; Polymers; Sintering; Surgery; Three dimensional; Transmission control protocol, Bioactive bones; Bioactive materials; Bioglass; Bioinert; Bone formations; Bone substitutes; Cell morphologies; Cell viabilities; Control groups; Human osteoblasts; In vitro; In-vivo; Laser sintering; Polyetheretherketone; Polyetheretherketone composites; Polymer powders; Reconstructive surgeries; Tricalcium phosphates, Bioactive glass, biomaterial; calcium phosphate; carbon; glass; nanoparticle; polyetheretherketone; polymer; unclassified drug, article; bone conduction; bone prosthesis; cell proliferation; cell structure; cell viability; composite material; controlled study; human; human cell; in vitro study; laser; osteoblast, Biocompatible Materials; Calcium Phosphates; Cell Proliferation; Cell Shape; Cell Survival; Cells, Cultured; Glass; Humans; Ketones; Lasers; Materials Testing; Osteoblasts; Polyethylene Glycols
