Τίτλος:
CHARACTERIZATION OF THE CHROMOSOMAL AAC(6')-IC GENE FROM
SERRATIA-MARCESCENS
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The DNA sequence of the chromosomal aac(6’)-k gene from Serratia
marcescens, which had been previously cloned (H. M. Champion, P. M.
Bennett, D. A. Lewis, and D. S. Reeves, J. Antimicrob. Chemother.
22:587-596, 1988) was determined. High-pressure liquid chromatographic
analysis of extracts prepared from Escherichia coli carrying the
chromosomal aac(6’)-Ic gene on a plasmid confirmed the presence of
6’-N-acetyltransferase activity in this strain, which was suggested by
the aminoglycoside resistance profile. DNA sequence analysis of the
cloned 2,057-bp PstI fragment revealed several regions of homology to
previously characterized sequences from GenBank, including the rpoD and
tRNA-2 genes of E. coli. Subcloning experiments confirmed the coding
sequence of the aac(6’)-Ic gene to be at positions 1554 to 1992. The
predicted amino acid sequence of the AAC(6’)-Ic protein suggested that
it was the third member of a family of AAC(6’) proteins which included a
coding region identified between the aadB and aadA genes of Tn4000 and
an AAC(6’) protein encoded by pUO490, which was isolated from
Enterobacter cloacae. Primer extension analysis suggested that the -35
region of the aac(6’)-Ic promoter overlapped a large palindromic
sequence which may be involved in the regulation of the aac(6’)-Ic gene.
Hybridization experiments utilizing a restriction fragment from the
aac(6’)-Ic gene showed that all S. marcescens organisms carried this
gene whether or not the AAC(6’)-I resistance profile was expressed.
Organisms other than Serratia spp. did not hybridize to this probe.
Συγγραφείς:
SHAW, KJ
RATHER, PN
SABATELLI, FJ
MANN, P
MUNAYYER, H
and MIERZWA, R
PETRIKKOS, GL
HARE, RS
MILLER, GH and
BENNETT, P
DOWNEY, P
Περιοδικό:
Antimicrobial Agents and Chemotherapy
Εκδότης:
AMER SOC MICROBIOLOGY
DOI:
10.1128/AAC.36.7.1447
