Τίτλος:
1994 MCBRYDE-MEDAL-AWARD-LECTURE - INVESTIGATIONS OF ORGANIZED MONOLAYER
FILMS FOR BIOSENSOR DEVELOPMENT
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Our interests have focused on the investigation and development of
biosensors that use chemically selective membranes to measure the
concentration of specific species in complex media. One fundamental idea
is that protein, which can bind selectively to a specific organic or
biochemical species, can be incorporated into an ordered lipid or
surfactant membrane assembly such that selective binding events lead to
changes in the structure of the membrane (transduction) that can be
measured quantitatively. The primary advantage of this method of
detection is that it is applicable to interactions of enzymes,
antibodies, receptors, and lectins, and it may be extended to
investigations of DNA/RNA hybridization. This detection method therefore
provides a sensitive generic strategy for sensor applications. The
central problem to be solved is how the alteration of the structure of a
membrane that is caused by binding events of protein or genetic material
can give rise to an analytical signal. We have been focusing our efforts
in the areas of fluorescence spectroscopy and electrochemical methods.
The electrochemical methods rely on detection in changes of the
permeability of membranes to ions and provide systems with very low
background signal, leading to the possibility of detection of single
molecular-binding events. Fluorescent systems operate on the basis that
a chemically selective membrane containing a fluorescent indicator can
provide an analytical signal caused by the change of the structure of
the membrane due to the binding events.
Συγγραφείς:
KRULL, UJ
HEIMLICH, MS
KALLURY, KMR
PIUNNO, PAE
BRENNAN,
JD
BROWN, RS
NIKOLELIS, DP
Περιοδικό:
CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE
Εκδότης:
NATL RESEARCH COUNCIL CANADA
Λέξεις-κλειδιά:
FLUORESCENCE; LIPID MEMBRANE; ELECTROCHEMISTRY; RECEPTOR; BIOSENSOR
