Τίτλος:
MOLECULAR AND IMMUNOHISTOCHEMICAL STUDY OF CLASS-I GROWTH-FACTOR
RECEPTORS IN SQUAMOUS-CELL LUNG CARCINOMAS
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The class I growth factor receptor family includes epidermal growth
factor receptor, i.e. c-erbB-1, c-erbB-2 and c-erbB-3 molecules. These
receptors have a significant sequence homology and play an important
role in cell growth and differentiation. To further investigate their
implication in squamous cell lung carcinomas (SqCLCs), we studied the
protein expression by immunohistochemistry and examined for possible
gene amplification by a novel semi-quantitative differential polymerase
chain reaction (DPCR) technique. Expression of c-erbB-1, c-erbB-2 and
c-erbB-3 was present in 65%, 28% and 10% respectively, of 40 SqCLCs
cases. Seven of the 11 cases that expressed c-erbB-2, was well as all 4
c-erbB-3 expressing cases, also stained with the anti-c-erbB-1 mAb.
Expression of c-erbB-1, but not c-erbB-2 or c-erbB-3, correlated with
the grade of tumor differentiation (100%, 64% and 36% positive cases
of well, moderately and poorly differentiated cases respectively, p <
0.003). In addition, c-erbB-1 expression correlated with the presence of
regional lymph node metastases within the moderately differentiated
group. The c-erbB-1 gene was amplified in 11/40 (28%) cases, all of
which overexpressed c-erbB-1 protein, while c-erbB-2 gene amplification
was detected in only one case. There was no c-erbB-3 gene amplification
in any of the 40 SqCLCs cases. These findings suggest that c-erbB-1,
c-erbB-2 and c-erbB-3 receptors do not have a common role and are of
different physiological importance, at least at the stage of clinically
overt tumor in human SqCLCs.
Συγγραφείς:
GORGOULIS, V
SFIKAKIS, PP
KARAMERIS, A
PAPASTAMATIOU, H and
TRIGIDOU, R
VESLEMES, M
SPANDIDOS, DA
SFIKAKIS, P and
JORDANOGLOU, J
Περιοδικό:
PATHOLOGY RESEARCH AND PRACTICE
Εκδότης:
ELSEVIER GMBH, URBAN & FISCHER VERLAG
Λέξεις-κλειδιά:
C-ERBB-1; C-ERBB-2; C-ERBB-3; SQUAMOUS CELL LUNG CARCINOMA; GENE
AMPLIFICATION; IMMUNOHISTOCHEMISTRY
DOI:
10.1016/S0344-0338(11)80595-9
