Τίτλος:
KLEBSIELLA-PNEUMONIAE INFECTIONS IN GREEK HOSPITALS - DISSEMINATION OF
PLASMIDS ENCODING AN SHV-5 TYPE BETA-LACTAMASE
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
A total of 160 Klebsiella pneumoniae clinical strains consecutively
isolated in 14 Greek hospitals in a three-month period was examined.
Application of capsular typing using 72 monovalent antisera combined
with phage-typing using a set of 15 Klebsiella-specific phages showed
the absence of epidemic strains. However, 41% of the isolates examined
displayed high level resistance to ceftazidime and aztreonam and, in
most of the cases, to more than one aminoglycoside as well as to other
antibacterial drugs. Nearly all of these multi-resistant strains were
epidemiologically distinct on the basis of their capsular serotype and
phage reactivity. After examination of 14 distinct strains, it was found
that in nine cases the resistance characters were readily transferred to
Escherichia coli recipients through large self-transmissible plasmids
(15-100 MDa). Six of the nine plasmids had equal molecular weight (60
MDa) and displayed similar fragment profiles upon digestion with
restriction endonuclease EcoRI. Isoelectric focusing and hydrolytic
studies showed that the prominent beta-lactamase produced by the
transconjugants harbouring the 60 MDa plasmids and the respective K.
pneumoniae parent strains, was an extended-spectrum beta-lactamase of
the SHV-5 type. It appears that among K. pneumoniae strains isolated in
Greek hospitals a significant resistance rate to both newer beta-lactams
and aminoglycosides has been established through the acquisition of
promiscuous multi-resistant plasmids which share a high degree of
similarity.
Συγγραφείς:
LEGAKIS, NJ
TZOUVELEKIS, LS
HATZOUDIS, G
TZELEPI, E and
GOURKOU, A
PITT, TL
VATOPOULOS, AC
Περιοδικό:
JOURNAL OF HOSPITAL INFECTION
Εκδότης:
W B SAUNDERS CO LTD
Λέξεις-κλειδιά:
KLEBSIELLA PNEUMONIAE; TYPING; PLASMIDS; BETA-LACTAMASE
DOI:
10.1016/0195-6701(95)90064-0
