Τίτλος:
Regulation of corticotropin releasing hormone receptor type 1 messenger
RNA level in Y-79 retinoblastoma cells: Potential implications for human
stress response and immune/inflammatory reaction
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
WE report the regulation of type 1 receptor mRNA in Y-79 human
retinoblastoma cells, grown in the absence or presence of
pharmacological levels of phorbol esters, forskolin, glucocorticoids and
their combinations. To control for inducibility and for assessing the
sensitivity of the Y-79 system to glucocorticoids, corticotropin
releasing hormone mRNA levels were measured in parallel. All treatments
stimulated corticotropin releasing hormone receptor type I gene
expression relative to baseline. A weak suppression of corticotropin
releasing hormone mRNA level was observed during dexamethasone
treatment. The cell line expressed ten-fold excess of receptor to Ligand
mRNA under basal conditions. The findings predict the presence of
functional phorbol ester, cyclic AMP and glucocorticoid response
elements in the promoter region of corticotropin releasing hormone
receptor type I gene and support a potential role for its product during
chronic stress and immune/inflammatory reaction.
Συγγραφείς:
Vamvakopoulos, NC
Sioutopoulou, TO
Mamuris, Z
Marcoulatos, P
and Avgerinos, PC
Περιοδικό:
Mediators of Inflammation
Εκδότης:
RAPID SCIENCE PUBLISHERS
Λέξεις-κλειδιά:
forskolin; glucocorticoids; homeostasis; human corticotropin releasing
hormone receptor; hypothalamic-pituitary-adrenal axis;
immune/inflammatory reaction; messenger RNA induction; phorbol esters;
stress response; Y-79 human retinoblastoma cells
DOI:
10.1155/S0962935196000476
