Τίτλος:
Prevalence of Kaposi's sarcoma associated herpesvirus infection measured
by antibodies to recombinant capsid protein and latent
immunofluorescence antigen
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background Kaposi’s sarcoma-associated herpesvirus (KSHV), also known as
human herpesvirus 8, may be the infectious cause of KS. Its prevalence
in the general population, on the basis of detection of the virus
genome, is controversial. To investigate the seroprevalence, we measured
antibodies to a recombinant capsid-related (lytic cycle) KSHV antigen
and a latent antigen complex.
Methods We selected potentially immunoreactive capsid-related proteins
of KSHV by expressing them as recombinant proteins and testing them in
western blot assays. We used a truncated recombinant protein encoded by
KSHV open reading frame 65 (orf 65) to develop a diagnostic
enzyme-linked immunosorbent assay (ELISA) and tested sera from
HIV-infected individuals with KS, HIV-uninfected patients with
‘’classic” KS, other HIV risk groups, and blood donors. We also
compared the antibody response to this capsid-related protein to the
response to latent antigen(s) in an immunofluorescence assay.
Findings 77/92 (84%) sera from KS patients reacted with the KSHV orf 65
protein and 84/103 (81 . 5%) reacted with KSHV latent antigen(s). The
dominant immunogenic region of orf 65 is within the carboxyterminal 80
aminoacids, a region with little sequence similarity to the related
Epstein-Barr virus, suggesting that orf 65 is a KSHV specific antigen.
Only three sera from patients with haemophilia (1/84) or from
intravenous drug users (2/63) had KSHV specific antibodies in the orf 65
assay whereas none of these sera reacted with latent antigen. Antibodies
to KSHV were also infrequently found in UK and US blood donors by either
assay (UK, 3/174 with orf 65 and 4/150 with latent antigen; US, 6/117
with orf 65 and 0/117 with latent antigen). They were more common among
HIV-infected gay men without KS (5/16 by orf 65 ELISA, 10/33 by IFA),
HIV-uninfected STD clinic attenders (14/166 by IFA), and Ugandan
HIV-uninfected controls (6/17 by orf 65 ELISA, 9/17 by IFA). Antibody
reactivity to the orf 65 protein (ELISA) and to latent antigen(s) (IFA)
was concordant in 89% of 462 sera tested but reactive blood donor sera
were discordant in both assays. Four AIDS-KS sera were unreactive in
both assays.
Interpretation The distribution of antibodies to both a capsid-related
recombinant protein and latent antigen(s) of KSHV strongly supports the
view that infection with this virus is largely confined to individuals
with, or at increased risk for, KS. However, infection with KSHV does
occur, rarely, in the general UK and US population and is more common in
Uganda. Antibodies to latent antigen(s) or to orf 65 encoded capsid
protein will not detect all cases of KSHV infection, and a combination
of several antigens will probably be required for accurate screening and
confirmatory assays.
Συγγραφείς:
Simpson, GR
Schulz, TF
Whitby, D
Cook, PM
Boshoff, C and
Rainbow, L
Howard, MR
Gao, SJ
Bohenzky, RA
Simmonds, P
and Lee, C
deRuiter, A
Hatzakis, A
Tedder, RS
Weller,
IVD
Weiss, RA
Moore, PS
Περιοδικό:
The Lancet Neurology
Εκδότης:
EXCERPTA MEDICA INC-ELSEVIER SCIENCE INC
DOI:
10.1016/S0140-6736(96)07560-5
