Τίτλος:
Direct amplification of minisatellite-region DNA with VNTR core
sequences in the genus Oryza
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
A polymerase chain reaction (PCR) application, involving the directed
amplification of minisatellite-region DNA (DAMD) with several
minisatellite core sequences as primers, was used to detect genetic
variation in 17 species of the genus Qryza and several rice cultivars
(O. sativa L.). The electrophoretic analysis of DAMD-PCR products showed
high levels of variation between different species and little variation
between different cultivars of O. sativa. Polymorphisms were also found
between accessions within a species, and between individual plants
within an accession of several wild species. The DAMD-PCR yielded
genome-specifie banding patterns for the species studied. Several
DAMD-PCR-generated DNA fragments were cloned and characterized, One
clone was capable of detecting multiple fragments and revealed
individual-specific hybridization banding patterns using genomic DNA
from wild species as well as rice cultivars. A second clone detected
only a single polymorphic locus, while a third clone expressed a strong
genome specificity by Southern analysis. The results demonstrated that
DAMD-PCR is potentially useful for species and genome identification in
Oryza. The DAMD-PCR technique also allows for the isolation of
informative molecular probes to be utilized in DNA fingerprinting and
genome identification in rice.
Συγγραφείς:
Zhou, Z
Bebeli, PJ
Somers, DJ
Gustafson, JP
Περιοδικό:
TAG Theoretical and Applied Genetics
Λέξεις-κλειδιά:
Oryza; rice; PCR; DNA fingerprinting; minisatellite
DOI:
10.1007/s001220050645
