Τίτλος:
Detection of human papillomaviruses in squamous cell carcinomas of the
lung
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The aim of this study was to evaluate the possible association of human
papillomaviruses (HPV) with the development of squamous cell lung
carcinomas (SqCLCs). Tissue material from 52 cases of SqCLCs were
studied, and the data were evaluated according to the degree of
differentiation, HPV presence and type. Analysis was performed by
polymerase chain reaction (PCR) method using consensus primers, and the
results were confirmed by subsequent Southern blot hybridization.
Overall, the results showed 69% positivity (n=32). Forty-one cases were
examined for the presence of specific HPV types (6/11 and 16/18) by
hybridization of the PCR products with P-32-labelled probes. HPV 6/11
types were detected in 6 of the 29 positive cases (20.6%). HPV 16/18
types were the most prevalent types, and were detected in 11/29 cases
(37.9%: 4/10 of well-differentiated cases, 6/25 of moderately and 1/6
of poorly differentiated carcinomas). Our results confirm the
possibility that HPV might play a role in the development of SqCLCs and
suggest a possible relation of high-risk HPV16/18 types to tumour
differentiation.
Συγγραφείς:
Papadopoulou, K
Labropoulou, V
Davaris, P
Mavromara, P and
Tsimara-Papastamatiou, H
Περιοδικό:
Virchows Archiv
Λέξεις-κλειδιά:
human papillomaviruses; squamous cell lung carcinoma; polymerase chain
reaction; Southern blot hybridization
DOI:
10.1007/s004280050215
