Τίτλος:
The effect of sclareol on growth and cell cycle progression of human
leukemic cell lines
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Sclareol, a labdane-type diterpene, was tested for cytotoxic effect
against a panel of established human leukemic cell lines. The compound
showed an IC50 lower than 20 mu g/ml in most cell lines tested, while it
was higher for resting peripheral blood mononuclear leukocytes (PBML).
Furthermore, the compound was tested for cytostatic activity against
four of the leukemic cell lines used. At a concentration of 20 mu g/ml
the compound showed a significant cytostatic effect as soon as 4 h after
continuous incubation against two from B and two from T lineage cell
lines. The morphology and the kind of death induced from sclareol in
three cell lines, was also investigated. The effect of sclareol on the
cell cycle progression of two cell lines, using flow cytometry, was
examined. The results show that sclareol kills cell lines, through the
process of apoptosis. The appearance of the apoptotic signs is time and
dose dependent. From the flow cytometry experiments, a delay of the cell
population on G(0/1) seems to take place. This is the first report, that
a labdane type diterpene kills tumor cells via a phase specific
mechanism which induces apoptosis. (C) 1999 Elsevier Science Ltd. All
rights reserved.
Συγγραφείς:
Dimas, K
Kokkinopoulos, D
Demetzos, C
Vaos, B
Marselos,
M
Malamas, M
Tzavaras, T
Περιοδικό:
Leukemia Research
Εκδότης:
PERGAMON-ELSEVIER SCIENCE LTD
Λέξεις-κλειδιά:
apoptosis; sclareol; human leukemic cell lines; cytotoxic/cytostatic
effect; cell cycle
DOI:
10.1016/S0145-2126(98)00134-9
