Τίτλος:
Cytotoxic and antiproliferative effects of heptaacetyltiliroside on
human leukemic cell lines
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The peracetylated derivative of kaempferol-3-O-beta-D-(6
“-E-p-coumaroyl) glycopyranoside (tiliroside) (1a) was tested for its
cytotoxic and cytostatic activity against several human leukemic cell
lines. The significant cytotoxic activity of this derivative, prompted
to an additional examination on some of the cell lines used. The effect
on the uptake of [H-3]thymidine as a marker of DNA synthesis and on
the cell proliferation, was investigated as well as the morphology of
the cells and the kind of death induced, using the Wright-Giemsa dye and
horizontal agarose-gel electrophoresis. Flow cytometric experiments of
1a on some leukemic cell lines was also performed. Compound 1a showed a
significant antiproliferative effect as soon as 1 h of continuous
incubation at all cell lines tested. Cells were killed, through the
process of apoptosis and the appearance of the apoptotic signs was time
and dose-dependent, while from the flow cytometric experiments, a
synchronisation (through a delay probably in the G(0/1) phase) of the
cells seems to take place. (C) 1999 Published by Elsevier Science Ltd.
All rights reserved.
Συγγραφείς:
Dimas, K
Demetzos, C
Vaos, B
Marselos, M
Kokkinopoulos,
D
Περιοδικό:
Leukemia Research
Εκδότης:
PERGAMON-ELSEVIER SCIENCE LTD
Λέξεις-κλειδιά:
tiliroside; human leukemic cell lines; apoptosis; cytotoxic/cytostatic
effect
DOI:
10.1016/S0145-2126(99)00124-1
