Τίτλος:
Localization of glucocorticoid hormone receptors in mitochondria of
human cells
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Glucocorticoid hormones regulate the transcription of nuclear genes by
may of their cognate receptors. In addition, these hormones also
modulate mitochondrial gene transcription by mechanisms which are as yet
poorly understood. Using immunofluorescence labeling and confocal laser
scanning microscopy we show that the glucocorticoid receptor of HeLa and
Hep-2 cells is specifically enriched at the sites of the mitochondria
which were visualized by labeling with the vital dye CMX and antibodies
against cytochrome oxidase subunit I. Immunogold electron microscopy
demonstrated that the receptor was located within the inner space of the
mitochondria, Immunoblotting experiments also revealed the presence of
glucocorticold receptor in mitochondria isolated from HeLa and Hep-2
cells. Finally living HeLa cells expressing green
fluorescentglucocorticoid receptor fusion protein revealed a distinct
mitochondrial GFP fluorescence. Our results support the concept of a
receptor-mediated direct action of steroid hormones on mitochondrial
gene transcription.
Συγγραφείς:
Scheller, K
Sekeris, CE
Krohne, G
Hock, R
Hansen, IA and
Scheer, U
Περιοδικό:
European Journal of Cell Biology
Εκδότης:
ELSEVIER GMBH, URBAN & FISCHER VERLAG
Λέξεις-κλειδιά:
glucocorticoid receptor; mitochondria; immunolocalization; green
fluorescent protein; HeLa cells; Hep-2 cells
DOI:
10.1078/S0171-9335(04)70033-3
