Περίληψη:
Rapid and efficient epidemiologic typing systems would be useful to
monitor transmission of methicillin-resistant Staphylococcus aureus
(MRSA) at both local and interregional levels. To evaluate the
intralaboratory performance and interlaboratory reproducibility of three
recently developed repeat-element PCR (rep-PCR) methods for the typing
of MRSA, 50 MRSA strains characterized by pulsed-field gel
electrophoresis (PFGE) (SmaI) analysis and epidemiological data were
blindly typed by inter-IS256, 16S-23S ribosomal DNA (rDNA), and MP3 PCR
in 12 laboratories in eight countries using standard reagents and
protocols. Performance of typing was defined by reproducibility (R),
discriminatory power (D), and agreement with PFGE analysis.
Interlaboratory reproducibility of pattern and type classification was
assessed visually and using gel analysis software. Each typing method
shelved a different performance level in each center. In the center
performing best with each method, inter-IS256 PCR typing achieved R =
100% and D = 100%; 16S-23S rDNA PCR, R = 100% and D = 82%; and MP3
PCR, R = 80% and D = 83%. Concordance between rep-PCR type and PFGE
type ranged by center: 70 to 90% for inter-IS256 PCR, 44 to 57% for
16S-23S rDNA PCR, and 53 to 54% for MP3 PCR analysis. In conclusion,
the performance of inter-IS256 PCR typing was similar to that of PFGE
analysis in some but not all centers, whereas other rep-PCR protocols
showed lower discrimination and intralaboratory reproducibility. None of
these assays, however, was sufficiently reproducible for interlaboratory
exchange of data.
Συγγραφείς:
Deplano, A
Schuermans, A
van Eldere, J
Witte, W and
Meugnier, H
Etienne, J
Grundmann, H
Jonas, D
Noordhoek,
GT
Dijkstra, J
van Belkum, A
van Leeuwen, W
Tassios, PT
and Legakis, NJ
van der Zee, A
Bergmans, A
Blanc, DS and
Tenover, FC
Cookson, BC
O'Neil, G
Struelens, MJ
European
Study Grp Epidemiological
