Τίτλος:
A comparative study of immunocapture ELISA and RT-PCR for screening
clinical samples from Southern Greece for human influenza virus types A
and B
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
An immunocapture (IC) ELISA and reverse transcriptase (RT)-PCR assays
were evaluated as screening methods for the detection of influenza virus
types A and B in clinical samples collected from individuals presenting
with influenza-like symptoms in Southern Greece. Standard virus
isolation in embryonated hens’ eggs was taken as the reference method.
According to the reference method, 25 (16.7%) of the 150 clinical
samples examined were infected by influenza viruses - 19 type A (H3N2)
and 6 type B, The sensitivity of immunocapture ELISA was 64% and that
for RT-PCR was 100%, The specificity of IC ELISA was 98% and by RT-PCR
97%, The positive diagnostic value of IC ELISA was 94% and of RT-PCR
86%, whereas the negative diagnostic values for IC ELISA and PCR were
93% and 100%, respectively. These findings confirm that RT-PCR
provides significantly increased sensitivity over IC ELISA and can be of
value in the management of regional influenza screening surveys
conducted by the national public health services.
Συγγραφείς:
Plakokefalos, E
Markoulatos, P
Ktenas, E
Spyrou, N and
Vamvakopoulos, NC
Περιοδικό:
Journal of Medical Microbiology
Εκδότης:
Lippincott, Williams & Wilkins
DOI:
10.1099/0022-1317-49-11-1037
