Τίτλος:
Accurate SARS-CoV-2 seroprevalence surveys require robust multi-antigen assays
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
There is a plethora of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) serological tests based either on nucleocapsid phosphoprotein (N), S1-subunit of spike glycoprotein (S1) or receptor binding domain (RBD). Although these single-antigen based tests demonstrate high clinical performance, there is growing evidence regarding their limitations in epidemiological serosurveys. To address this, we developed a Luminex-based multiplex immunoassay that detects total antibodies (IgG/IgM/IgA) against the N, S1 and RBD antigens and used it to compare antibody responses in 1225 blood donors across Greece. Seroprevalence based on single-antigen readouts was strongly influenced by both the antigen type and cut-off value and ranged widely [0.8% (95% CI 0.4–1.5%)–7.5% (95% CI 6.0–8.9%)]. A multi-antigen approach requiring partial agreement between RBD and N or S1 readouts (RBD&N|S1 rule) was less affected by cut-off selection, resulting in robust seroprevalence estimation [0.6% (95% CI 0.3–1.1%)–1.2% (95% CI 0.7–2.0%)] and accurate identification of seroconverted individuals. © 2021, The Author(s).
Συγγραφείς:
Fotis, C.
Meimetis, N.
Tsolakos, N.
Politou, M.
Akinosoglou, K.
Pliaka, V.
Minia, A.
Terpos, E.
Trougakos, I.P.
Mentis, A.
Marangos, M.
Panayiotakopoulos, G.
Dimopoulos, M.A.
Gogos, C.
Spyridonidis, A.
Alexopoulos, L.G.
Περιοδικό:
Scientific Reports
Εκδότης:
Institute of Geographic Sciences and Natural Resources Research
Λέξεις-κλειδιά:
antigen; coronavirus spike glycoprotein; immunoglobulin A; immunoglobulin G; immunoglobulin M; nucleocapsid phosphoprotein, SARS-CoV-2; phosphoprotein; spike protein, SARS-CoV-2; virus antibody, adolescent; adult; aged; blood; diagnosis; female; human; immunoassay; immunology; isolation and purification; male; middle aged; procedures; sensitivity and specificity; serology; virology; young adult, Adolescent; Adult; Aged; Antibodies, Viral; Antigens; Coronavirus Nucleocapsid Proteins; COVID-19; Female; Humans; Immunoassay; Immunoglobulin A; Immunoglobulin G; Immunoglobulin M; Male; Middle Aged; Phosphoproteins; SARS-CoV-2; Sensitivity and Specificity; Serologic Tests; Spike Glycoprotein, Coronavirus; Young Adult
DOI:
10.1038/s41598-021-86035-2
