Τίτλος:
Conditioning attenuates kidney and heart injury in rats following transient suprarenal occlusion of the abdominal aorta
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Suprarenal aortic clamping during abdominal aortic aneurysm (AAA) repair results in ischemia-reperfusion injury (IRI) in local (i.e. kidney) and distant (i.e. heart) tissue. To investigate perioperative approaches that mitigate IRI-induced tissue damage, Wistar rats underwent suprarenal aortic clamping either alone or in combination with short cycles of ischemic conditioning before and/or after clamping. Serum analysis revealed significant reduction in key biochemical parameters reflecting decreased tissue damage at systemic level and improved renal function in conditioned groups compared to controls (p < 0.05), which was corroborated by histolopathological evaluation. Importantly, the levels of DNA damage, as reflected by the biomarkers 8-oxo-G, γH2AX and pATM were reduced in conditioned versus non-conditioned cases. In this setting, NADPH oxidase, a source of free radicals, decreased in the myocardium of conditioned cases. Of note, administration of 5-HD and 8-SPT blocking key protective signaling routes abrogated the salutary effect of conditioning. To further understand the non-targeted effect of IRI on the heart, it was noted that serum TGF-β1 levels decreased in conditioned groups, whereas this difference was eliminated after 5-HD and 8-SPT administration. Collectively, conditioning strategies reduced both renal and myocardial injury. Additionally, the present study highlights TGF-β1 as an attractive target for manipulation in this context. © 2020, The Author(s).
Συγγραφείς:
Karageorgiadi, D.M.
Tsilimigras, D.I.
Selemenakis, P.
Vlachou, V.
de Lastic, A.-L.
Rodi, M.
Chatziathanasiou, D.
Savvatakis, K.
Antoniou, N.
Deli, A.C.
Papalampros, A.
Filis, K.A.
Mouzaki, A.
Varvarigou, A.
Zografos, G.
Gorgoulis, V.G.
Pateras, I.S.
Sigala, F.
Περιοδικό:
Scientific Reports
Εκδότης:
Institute of Geographic Sciences and Natural Resources Research
Λέξεις-κλειδιά:
reduced nicotinamide adenine dinucleotide phosphate oxidase; transforming growth factor beta1, abdominal aorta; abdominal aortic aneurysm; acute kidney failure; adverse event; animal; DNA damage; genetics; ischemic preconditioning; ligation; male; metabolism; myocardial ischemia reperfusion injury; procedures; reperfusion injury; vascular surgery; Wistar rat, Acute Kidney Injury; Animals; Aorta, Abdominal; Aortic Aneurysm, Abdominal; Constriction; DNA Damage; Ischemic Preconditioning; Male; Myocardial Reperfusion Injury; NADPH Oxidases; Rats, Wistar; Reperfusion Injury; Transforming Growth Factor beta1; Vascular Surgical Procedures
DOI:
10.1038/s41598-020-61268-9
