Τίτλος:
HIV-1 transmission networks across cyprus (2010-2012)
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
A molecular epidemiology study of HIV-1 infection was conducted in one hundred diagnosed and untreated HIV-1-infected patients in Cyprus between 2010 and 2012, representing 65.4% of all the reported HIV-1 infections in Cyprus in this three-year period, using a previously defined enrolment strategy. Eighty-two patients were newly diagnosed (genotypic drug resistance testing within six months from diagnosis), and eighteen patients were HIV-1 diagnosed for a longer period or the diagnosis date was unknown. Phylogenetic trees of the pol sequences obtained in this study with reference sequences indicated that subtypes B and A1 were the most common subtypes present and accounted for 41.0 and 19.0% respectively, followed by subtype C (7.0%), F1 (8.0%), CRF02_AG (4.0%), A2 (2.0%), other circulating recombinant forms (CRFs) (7.0%) and unknown recombinant forms (URFs) (12%). Most of the newly-diagnosed study subjects were Cypriots (63%), males (78%) with median age 39 (Interquartile Range, IQR 33–48) reporting having sex with other men (MSM) (51%). A high rate of clustered transmission of subtype B drug-sensitive strains to reverse transcriptase and protease inhibitors was observed among MSM, twenty-eight out of forty-one MSM study subjects (68.0%) infected were implicated in five transmission clusters, two of which are sub-subtype A1 and three of which are subtype B strains. The two largest MSM subtype B clusters included nine and eight Cypriot men, respectively, living in all major cities in Cyprus. There were only three newly diagnosed patients with transmitted drug resistant HIV-1 strains, one study subject from the United Kingdom infected with subtype B strain and one from Romania with sub-subtype A2 strain, both with PI drug resistance mutation M46L and one from Greece with sub-subtype A1 with nonnucleoside reverse transcriptase inhibitors (NNRTI) drug resistance mutation K103N. © 2018 Kostrikis et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Συγγραφείς:
Kostrikis, L.G.
Hezka, J.
Stylianou, D.C.
Kostaki, E.
Andreou, M.
Kousiappa, I.
Paraskevis, D.
Demetriades, I.
Εκδότης:
Public Library of Science
Λέξεις-κλειδιά:
asparagine; lysine; nonnucleoside reverse transcriptase inhibitor; proteinase inhibitor; RNA directed DNA polymerase inhibitor, adult; antiviral resistance; Article; clinical article; Cypriot; Cyprus; female; gene sequence; genotype; Greece; human; human cell; Human immunodeficiency virus 1; Human immunodeficiency virus 1 infection; male; men who have sex with men; molecular epidemiology; nonhuman; phylogenetic tree; prospective study; Romania; structural gene; United Kingdom; urban area; virus mutation; virus recombinant; virus strain; virus transmission; adolescent; aged; epidemiology; genetics; Human immunodeficiency virus infection; male homosexuality; medical geography; middle aged; molecular epidemiology; phylogeny; transmission; young adult, Adolescent; Adult; Aged; Cyprus; Drug Resistance, Viral; Female; Geography, Medical; HIV Infections; HIV-1; Homosexuality, Male; Humans; Male; Middle Aged; Molecular Epidemiology; Phylogeny; Prospective Studies; Young Adult
DOI:
10.1371/journal.pone.0195660
