Τίτλος:
Urine metabolomics in neonates with late-onset sepsis in a case-control study
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Although late-onset sepsis (LOS) is a major cause of neonatal morbidity and mortality, biomarkers evaluated in LOS lack high diagnostic accuracy. In this prospective, case-control, pilot study, we aimed to determine the metabolic profile of neonates with LOS. Urine samples were collected at the day of initial LOS evaluation, the 3 rd and 10 th day, thereafter, from 16 septic neonates (9 confirmed and 7 possible LOS cases) and 16 non-septic ones (controls) at respective time points. Urine metabolic profiles were assessed using non-Targeted nuclear magnetic resonance spectroscopy and targeted liquid chromatography-Tandem mass spectrometry analysis. Multivariate statistical models with data from either analytical approach showed clear separation between the metabolic profiles of septic neonates (both possible and confirmed) and the controls. Metabolic changes appeared to be related to disease progression. Overall, neonates with confirmed or possible LOS exhibited comparable metabolic profiles indicating similar metabolic alternations upon the onset of clinical manifestations. This methodology therefore enabled the discrimination of neonates with LOS from non-septic individuals, providing potential for further research toward the discovery of LOS-related biomarkers. © The Author(s) 2017.
Συγγραφείς:
Sarafidis, K.
Chatziioannou, A.C.
Thomaidou, A.
Gika, H.
Mikros, E.
Benaki, D.
Diamanti, E.
Agakidis, C.
Raikos, N.
Drossou, V.
Theodoridis, G.
Περιοδικό:
Scientific Reports
Εκδότης:
Nature Publishing Group
Λέξεις-κλειδιά:
biological marker, case control study; chemistry; human; late onset disorder; liquid chromatography; metabolomics; newborn; newborn sepsis; nuclear magnetic resonance spectroscopy; pathology; pilot study; prospective study; tandem mass spectrometry; urinalysis; urine, Biomarkers; Case-Control Studies; Chromatography, Liquid; Humans; Infant, Newborn; Late Onset Disorders; Magnetic Resonance Spectroscopy; Metabolomics; Neonatal Sepsis; Pilot Projects; Prospective Studies; Tandem Mass Spectrometry; Urinalysis; Urine
