Τίτλος:
Field application of SD bioline malaria Ag Pf/ pan rapid diagnostic test for malaria in Greece
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Greece, a malaria-free country since 1974, has experienced re-emergence of Plasmodium vivax autochthonous malaria cases in some agriculture areas over the last three years. In early 2012, an integrated control programme (MALWEST Project) was launched in order to prevent re-establishment of the disease. In the context of this project, the rapid diagnostic tests (RDT) of SD Bioline Malaria Ag Pf/Pan that detects hrp-2 and pan-LDH antigens were used. The aim of this study was to assess the field application of the RDT for the P. vivax diagnosis in comparison to light microscopy and polymerase chain reaction (PCR). A total of955 samples were tested with all three diagnostic tools. Agreement of RDT against microscopy and PCR for the diagnosis of P. vivax was satisfactory (K value: 0.849 and 0.976, respectively). The sensitivity, specificity and positive predictive value of RDT against PCR was 95.6% (95% C.I.: 84.8-99.3), 100% (95% C.I.: 99.6-100.0) and 100% (95% CI: 91.7- 100.0) respectively, while the sensitivity, specificity and positive predictive value of RDT against microscopic examination was 97.4%(95% C.I.: 86.1-99.6), 99.4% (95% C.I.: 98.6- 99.8) and 86.1%(95% CI: 72.1-94.7), respectively. Our results indicate that RDT performed satisfactory in a non-endemic country and therefore is recommended for malaria diagnosis, especially in areas where health professionals lack experience on light microscopy. © 2015 Tseroni et al.
Συγγραφείς:
Tseroni, M.
Pervanidou, D.
Tserkezou, P.
Rachiotis, G.
Pinaka, O.
Baka, A.
Georgakopoulou, T.
Vakali, A.
Dionysopoulou, M.
Terzaki, I.
Marka, A.
Detsis, M.
Evlampidou, Z.
Mpimpa, A.
Vassalou, E.
Tsiodras, S.
Tsakris, A.
Kremastinou, J.
Hadjichristodoulou, C.
Εκδότης:
Public Library of Science
Λέξεις-κλειδιά:
adolescent; adult; aged; Article; child; diagnostic test accuracy study; field study; Greece; human; major clinical study; malaria rapid test; microscopy; Plasmodium vivax malaria; polymerase chain reaction; predictive value; sensitivity and specificity; diagnostic test; economics; evaluation study; infant; isolation and purification; Malaria, Vivax; middle aged; Plasmodium vivax; preschool child; procedures; very elderly; young adult, Plasmodium vivax, parasite antigen, Adolescent; Adult; Aged; Aged, 80 and over; Antigens, Protozoan; Child; Child, Preschool; Diagnostic Tests, Routine; Greece; Humans; Infant; Malaria, Vivax; Microscopy; Middle Aged; Plasmodium vivax; Polymerase Chain Reaction; Sensitivity and Specificity; Young Adult
DOI:
10.1371/journal.pone.0120367
