Τίτλος:
Effect of cytostatic drugs on the mRNA expression levels of ribonuclease k in breast and ovarian cancer cells
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Breast and ovarian cancers remain a major public health issue, constituting a major cause of female morbidity and mortality worldwide. Even though systemic chemotherapy remains the main course of action for both types of cancer, current research studies aim at the discovery of novel therapeutic targets. Ribonucleases, due to their apparent implication in gene expression regulation, may serve as potential anticancer drugs. Human RNase κ is an endoribonuclease belonging in a recently identified family of proteins. Recent data from expression microarray studies reveal that the RNase κ gene is found either up- or down regulated in a number of human cancers, indicating a possible diagnostic and/or prognostic utility. The aim of this study was to investigate modulations in the expression levels of RNase κ gene, in breast and ovarian cancer cells, as a response to treatment with different chemotherapeutic agents. BT-20 breast cancer cells and SKOV-3 ovarian cancer cells were treated with the cytotoxic drugs paclitaxel, docetaxel, cisplatin, carboplatin, epirubicin and vinorelbine. Gene expression analysis was performed by the comparative CT method also known as 2-ΔΔCT method. The results revealed a distinct increase in the expression levels of RNase κ mRNA (up to 9-fold) after treatment with the antineoplastic agent paclitaxel in both cell lines, while treatment with the remaining anticancer drugs did not alter drastically the mRNA levels of RNase κ. Based on the fact that paclitaxel exerts its cytotoxic action by inducing apoptosis, the results could be indicative of a potential implication of RNase κ in apoptosis-related pathways. © 2014 Bentham Science Publishers.
Συγγραφείς:
Gkratsou, A.S.
Fragoulis, E.G.
Sideris, D.C.
Περιοδικό:
Anti-Cancer Agents in Medicinal Chemistry
Λέξεις-κλειδιά:
carboplatin; cisplatin; docetaxel; epirubicin; messenger RNA; navelbine; paclitaxel; ribonuclease; ribonuclease kappa; unclassified drug, article; breast cancer; cancer cell; cell culture; cell viability assay; controlled study; drug sensitivity; gene; gene expression; human; human cell; IC 50; ovary cancer; real time polymerase chain reaction; reverse transcription polymerase chain reaction; RNA extraction, Adenocarcinoma; Antineoplastic Agents; Breast Neoplasms; Cell Line, Tumor; Cytostatic Agents; Endoribonucleases; Female; Humans; Inhibitory Concentration 50; Ovarian Neoplasms; RNA, Messenger
DOI:
10.2174/18715206113139990090
