Τίτλος:
Effects of oral administration of L-arginine, L-NAME and allopurinol on intestinal ischemia/reperfusion injury in rats
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Aims: Intestinal ischemia/reperfusion (I/R) injury is implicated in many clinical conditions, and it performs a fundamental role in their pathophysiologies. Oral administration of antioxidants and nitric oxide (NO) donors ameliorate intestinal injury. Here, the effects of l-arginine, allopurinol and NG-nitro-l-arginine methyl ester (l-NAME) were investigated. Main methods: One hundred twenty-eight male Wistar rats were separated into 4 groups and subjected to occlusion of the superior mesenteric artery for 60 min. The Control group did not receive any substance before the surgical operation. However, the 3 other groups received the following: l-arginine (800 mg/kg body weight; l-Arg group), l-NAME (50 mg/kg; l-NAME group) or allopurinol (100 mg/kg; Allo group). Each substance was given by mouth in 3 equal doses 24, 12 and 1 h before the surgical operation. Each group was then divided into 4 subgroups, which underwent different durations of reperfusion (0, 1, 8 or 24 h). At the end of each time point, blood and tissue samples were collected, and histological examinations were performed. Serum nitrite and catalase, intestinal tissue myeloperoxidase (MPO), inducible nitric oxide synthase (iNOS) and nitrotyrosine (NT) levels were determined. Key findings: At each reperfusion time point, the Allo group exhibited the mildest histological lesions in contrast to the l-NAME group, which showed the most severe lesions. MPO was decreased significantly in the Allo and l-Arg groups during reperfusion, and allopurinol administration caused earlier and stronger effect. iNOS and NT levels were higher in the l-Arg group and lower in the Allo group. Serum nitrite and catalase were increased in the l-NAME group after 24 h. Significance: Oral administration of allopurinol exerted a strong and protective effect on the intestinal tissue that was subjected to I/R earlier than l-arginine. This finding was also supported with the MPO, iNOS and NT data. © 2011 Elsevier Inc. All rights reserved.
Συγγραφείς:
Margaritis, E.V.
Yanni, A.E.
Agrogiannis, G.
Liarakos, N.
Pantopoulou, A.
Vlachos, I.
Papachristodoulou, A.
Korkolopoulou, P.
Patsouris, E.
Kostakis, M.
Perrea, D.N.
Kostakis, A.
Περιοδικό:
Life Science Alliance
Λέξεις-κλειδιά:
3 nitrotyrosine; allopurinol; arginine; catalase; inducible nitric oxide synthase; myeloperoxidase; n(g) nitroarginine methyl ester; nitrite, animal experiment; animal model; animal tissue; article; controlled study; disease duration; enzyme activity; histopathology; intestine ischemia; male; nonhuman; pilot study; preoperative period; rat; reperfusion injury, Administration, Oral; Allopurinol; Animals; Arginine; Catalase; Intestines; Male; NG-Nitroarginine Methyl Ester; Nitric Oxide Synthase Type II; Nitrites; Peroxidase; Rats; Rats, Wistar; Reperfusion Injury; Time Factors; Tyrosine, Rattus; Rattus norvegicus
DOI:
10.1016/j.lfs.2011.04.009
