Τίτλος:
Effect of 7-O-β-D-glucopyranosylchrysin and its aglycone chrysin isolated from Podocytisus caramanicus on estrogen receptor α transcriptional activity
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
A screening of Greek Fabaceae extracts identified the methanolic extract of Podocytisus caramanicus Boiss. & Heldr. as having proliferative activity on human breast cancer cells (MCF-7). Using transient transfection experiments, we have first used three compounds described for their estrogen-like properties, E2, genistein (Gen) and biochanin A (Bch), as controls to evaluate our cellular model. Secondly, we have demonstrated that the 7-O-β-D- glucopyranosylchrysin (Glc-chr), the most abundant flavone of the extract, and its aglycone chrysin were able to increase estrogen receptor α transcriptional activity in MCF-7 cells. We have also shown that the estrogenic activity of Glc-chr could be completely suppressed by the pure estrogen antagonist ICI 182,780 suggesting that the effect of Glc-chr is mediated by ERα. © Georg Thieme Verlag KG Stuttgart.
Συγγραφείς:
Berthier, A.
Girard, C.
Grandvuillemin, A.
Muyard, F.
Skaltsounis, A.-L.
Jouvenot, M.
Delage-Mourroux, R.
Λέξεις-κλειδιά:
7 o beta glucopyranosylchrysin; aglycone chrysin; chrysin; estrogen receptor alpha; fulvestrant; plant extract; Podocytisus caramanicus extract; unclassified drug, antineoplastic activity; article; cell strain MCF 7; drug efficacy; estrogen activity; female; genetic transcription; genetic transfection; human; human cell; legume; medicinal plant; Podocytisus caramanicus, Antineoplastic Agents, Phytogenic; Breast Neoplasms; Cell Line, Tumor; Estrogen Receptor alpha; Fabaceae; Flavonoids; Glucosides; Humans; Molecular Structure; Transcription, Genetic, Fabaceae
DOI:
10.1055/s-2007-990248
